Leptin treatment rescues the sterility of genetically obese ob/ob males.
Leptin treatment rescues the sterility of genetically obese ob/ob males.
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DOI：
10.1210/endo.138.3.5024
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发表时间：
1997-03
期刊：
影响因子：
4.8
通讯作者：
K. Mounzih;R. Lu;Farid F. Chehab
中科院分区：
文献类型：
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作者：
K. Mounzih;R. Lu;Farid F. Chehab
瘦素是一种由白色脂肪组织分泌的激素，已被证明可以使ob/ob小鼠的体重正常化，但不能使db/db小鼠的体重正常化，这是Coleman在他的经典异种共生实验中所假设的。因此，瘦素的主要作用是减少食物摄入，从而导致脂肪储存的分解。最近，我们提出瘦素在生殖生理学中发挥作用，基于瘦素治疗而不是食物限制挽救了ob/ob女性的不育。在本次交流中，我们用瘦素治疗不育的ob/ob男性，询问是否可以诱导生育，因此选择他们的生殖能力作为实验的终点。我们的研究结果表明，所有食物限制的ob/ob雄性都不能使正常的C57BL/6J雌性受精。然而，所有接受瘦素治疗的雄性小鼠都能使正常怀孕和分娩的雌性小鼠受精，这表明只有瘦素治疗才能纠正雄性小鼠的生殖能力。此外，瘦素治疗动物的生殖指标如睾丸重量和组织学恢复正常。因此，在ob/ob雌性小鼠中，瘦素在雄性小鼠的生殖途径中起着重要作用。
Leptin, a hormone secreted from white adipose tissue, has been shown to normalize the body weight of ob/ob but not db/db mice as postulated by Coleman in his classical parabiosis experiments. The major effect of leptin is therefore to decrease food intake, thus resulting in a breakdown of fat stores. Recently, we have suggested that leptin plays a role in reproductive physiology based on the observation that leptin treatment but not food restriction rescues the sterility of ob/ob females. In the present communication, we treated sterile ob/ob males with leptin and asked whether fertility could be induced, thus selecting their reproductive ability as the endpoint of the experiment. Our results show that all food-restricted ob/ob males are unable to impregnate normal C57BL/6J females. However, all leptin-treated ob/ob males fertilized normal females mice that carried out normal pregnancies and deliveries, demonstrating that the reproductive capacity of ob/ob males was corrected only with leptin treatment. Furthermore, reproductive indices such as testicular weight and histology are normalized in leptin-treated animals. Therefore, as in ob/ob females, leptin plays a significant role in the male mouse reproductive pathways.