Analyzing Protein-Protein Interactions Using the Split-Ubiquitin System.
Analyzing Protein-Protein Interactions Using the Split-Ubiquitin System.
复制标题
使用分裂泛素系统分析蛋白质-蛋白质相互作用。
DOI：
10.1007/978-1-0716-3327-4_3
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Karnik R
中科院分区：
文献类型：
--
作者：
Karnik R
分裂泛素技术是20多年前开发的，作为基于Gal4的酵母双杂交方法的替代方法，用于识别相互作用的蛋白质伙伴。随着基于交配的分离泛素筛选方法的引入，该方法因其极高的转化效率、在处理全长膜蛋白方面的实用性以及几乎不受虚假相互作用干扰的正选择而广受欢迎。最近的进展将这些分裂泛素方法扩展到分析其他可溶蛋白质之间的相互作用和三方蛋白质之间的相互作用。
The split-ubiquitin technology was developed over 20 years ago as an alternative to Gal4-based, yeast-two-hybrid methods to identify interacting protein partners. With the introduction of mating-based methods for split-ubiquitin screens, the approach has gained broad popularity because of its exceptionally high transformation efficiency, utility in working with full-length membrane proteins, and positive selection with little interference from spurious interactions. Recent advances now extend these split-ubiquitin methods to the analysis of interactions between otherwise soluble proteins and tripartite protein interactions.