Development of a comparative genetic linkage map for Armigeres subalbatus using Aedes aegypti RFLP markers
Development of a comparative genetic linkage map for Armigeres subalbatus using Aedes aegypti RFLP markers
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DOI：
10.1101/gr.8.1.41
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发表时间：
1998-01-01
期刊：
影响因子：
7
通讯作者：
Christensen, BM
中科院分区：
文献类型：
--
作者：
Ferdig, MT;Taft, AS;Christensen, BM
淋巴丝虫病的病原体之一是马来布鲁氏线虫寄生虫，它的发展和传播需要一个称职的蚊子载体。亚信天蚊通过一种被称为黑素囊化的防御反应迅速摧毁入侵的马来芽孢杆菌微丝蚴。我们利用埃及伊蚊的RFLP标记构建了该蚊种的遗传连锁图谱。这种异源方法是可能的，因为作为标记的编码序列的保守性，并提供了一个实验框架来评估这些蚊子物种之间的连锁和基因顺序是保守的假设。在测试的56个埃及伊蚊标记中，77%在严格条件下与亚信天翁伊蚊的基因组DNA杂交，其中53%显示出菌株特异性多态性。利用源自日本和马来西亚的杂交菌株的f -2分离亚信天翁伊蚊种群，绘制了26个埃及伊蚊标记。这些标记位点的线性顺序在两个物种之间高度保守。这些标记中只有1个，LF92，没有按照Ae预测的方式连接。蚊地图。此外，常染色体性别决定位点发生在伊蚊的连锁组1。埃及伊蚊生活在第三群，亚信天翁。亚信天翁疟的图谱提供了一个基本的遗传背景，可以用于进一步的遗传研究，以阐明这种蚊子对寄生虫抗性的遗传基础，并且是鉴定携带决定包封表型基因的基因组区域的必要前提。
One of the causative agents of lympahtic filariasis is the nematode parasite Brugia malayi that requires a competent mosquito vector for its development and transmission. Armigeres subalbatus mosquitoes rapidly destroy invading B. malayi microfilariae via a defense response known as melanotic encapsulation. We have constructed a genetic linkage map for this mosquito species using RFLP markers from Aedes aegypti. This heterologous approach was possible because of the conserved nature of the coding sequences used as markers and provided an experimental framework to evaluate the hypothesis that linkage and gene order are conserved between these mosquito species. Of the 56 Ae, aegypti markers tested, 77% hybridize to genomic DNA digests of Ar. subalbatus under stringent conditions, with 53% of these demonstrating strain-specific polymorphisms. Twenty-six Ae, aegypti markers have been mapped using an F-2-segregating Ar. subalbatus population derived from a cross of strains originating in Japan and Malaysia. Linear order of these marker loci is highly conserved between the two species. Only 1 of these markers, LF92, was not linked in the manner predicted by the Ae. aegypti map. In addition, the autosomal sex-determination locus that occurs in linkage group 1 in Ae. aegypti resides in group 3 in Ar, subalbatus. The Ar. subalbatus map provides a basic genetic context that can be utilized in further genetic studies to clarify the genetic basis of parasite resistance in this mosquito and is a necessary precursor to the identification of genome regions that carry genes that determine the encapsulation phenotype.