Distribution and characterization of nitric oxide synthase in the nervous system of Triatoma infestans (Insecta: Heteroptera).
Distribution and characterization of nitric oxide synthase in the nervous system of Triatoma infestans (Insecta: Heteroptera).
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一氧化氮合酶在致病锥蝽（昆虫纲：异翅目）神经系统中的分布和特征。
DOI：
10.1007/s00441-006-0359-1
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发表时间：
2007
影响因子：
3.6
通讯作者：
Villar,MarceloJ
中科院分区：
文献类型：
--
作者：
Settembrini,BeatrizP;Coronel,MariaF;Nowicki,Susana;Nighorn,AlanJ;Villar,MarceloJ
研究了异翅蝽Triatoma infestans一氧化氮合酶（NOS）的生化特性及其在中枢神经系统（CNS）的分布。免疫细胞化学结果显示，NOS阳性细胞主要分布于脑、食管下神经节和胸神经节。在前脑中，NOS免疫反应（IR）胞体在前，侧，后索马皮检测。在视叶中，在第一视交叉水平、小叶周围和近端视叶中观察到许多免疫染色胞体。在中脑，NOS-IR胞体主要分布于外侧索马皮，包围感觉小球，少数细胞体与触角的机械感觉和运动神经元相连。在触角神经中未检测到免疫染色。食管下和胸前神经节含有散在的免疫染色细胞体。NOS-IR胞体存在于后神经节的所有神经节中。Western blotting显示，一种通用的NOS抗血清在134 kDa处识别一条带，与预期的蛋白质分子量一致。一氧化氮产生动力学分析显示，T的中枢神经系统组织样本中存在完全活性的酶。害虫
The biochemical characterization of nitric oxide synthase (NOS) and its distribution in the central nervous system (CNS) were studied in the heteropteran bugTriatoma infestans. NOS-like immunoreactivity was found in the brain, subesophageal ganglion, and thoracic ganglia by using immunocytochemistry. In the protocerebrum, NOS-immunoreactive (IR) somata were detected in the anterior, lateral, and posterior soma rinds. In the optic lobe, numerous immunostained somata were observed at the level of the first optic chiasma, around the lobula, and in the proximal optic lobe. In the deutocerebrum, NOS-IR perikarya were mainly observed in the lateral soma rind, surrounding the sensory glomeruli, and a few cell bodies were seen in association with the antennal mechanosensory and motor neuropil. No immunostaining could be detected in the antennal nerve. The subesophageal and prothoracic ganglia contained scattered immunostained cell bodies. NOS-IR somata were present in all the neuromeres of the posterior ganglion. Western blotting showed that a universal NOS antiserum recognized a band at 134 kDa, in agreement with the expected molecular weight of the protein. Analysis of the kinetics of nitric oxide production revealed a fully active enzyme in tissue samples of the CNS ofT. infestans.