INHIBITION OF NITRIC-OXIDE SYNTHESIS ENHANCES THE EXPRESSION OF INDUCIBLE NITRIC-OXIDE SYNTHASE MESSENGER-RNA AND PROTEIN IN A MODEL OF CHRONIC LIVER INFLAMMATION
INHIBITION OF NITRIC-OXIDE SYNTHESIS ENHANCES THE EXPRESSION OF INDUCIBLE NITRIC-OXIDE SYNTHASE MESSENGER-RNA AND PROTEIN IN A MODEL OF CHRONIC LIVER INFLAMMATION
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DOI：
10.1006/bbrc.1994.2506
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发表时间：
1994-10-28
影响因子：
3.1
通讯作者：
BILLIAR, TR
中科院分区：
文献类型：
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作者：
LUSS, H;DISILVIO, M;BILLIAR, TR
对感染性休克诱导型一氧化氮合酶（NOS）的抑制可能具有治疗价值。然而，副作用还有待研究。因此，我们在细小棒状杆菌（C. parvum）诱导的慢性肝脏炎症模型中研究了慢性NOS抑制对iNOS表达水平的影响，该模型导致肝脏中iNOS持续表达。NOS抑制剂使血浆亚硝酸盐/硝酸盐水平升高和尿排泄减少约50%；iNOS mRNA和蛋白含量分别增加了200%和150%。因此，在iNOS表达的情况下，长期抑制NOS可导致iNOS mRNA水平和蛋白水平升高。在去除nos抑制剂后，这可能导致NO的过量产生。(C) 1994学术出版社，Inc.
In septic shock the inhibition of inducible nitric oxide synthase (NOS) could be of therapeutic value. However, side effects have to be investigated. Therefore we studied the effects of chronic NOS inhibition on the level of iNOS expression in a model of chronic liver inflammation induced by Corynebacterium parvum (C. parvum) which causes sustained iNOS expression in the liver. NOS inhibitors decreased the rise in plasma levels and urinary excretion of nitrite/nitrate by about 50%; however, iNOS mRNA and protein were increased to 200% and 150%, respectively. Thus chronic inhibition of NOS can result in an increase in iNOS mRNA level and protein under conditions when iNOS is expressed. This could result in an overproduction of NO upon removal of the NOS-inhibitor. (C) 1994 Academic Press, Inc.