RBCs labeled at two biotin densities permit simultaneous and repeated measurements of circulating RBC volume
RBCs labeled at two biotin densities permit simultaneous and repeated measurements of circulating RBC volume
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DOI：
10.1111/j.1537-2995.2004.00654.x
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发表时间：
2004-03-01
期刊：
影响因子：
2.9
通讯作者：
Strauss, RG
中科院分区：
文献类型：
--
作者：
Mock, DM;Lankford, GL;Strauss, RG
BACKGROUND: The extend potential applications of a nonradioactive method for measuring circulating FBC volume, we tested the hypothesis that RBC volume could be determined independently using two populations of RBCs labeled with low-density biotin (LDB1) and high-density biotin (HDB).STUDY DESIGN AND METHODS: In 10 healthy adults, autologous RBCs were labeled with HDB, LDB, or Cr-51. The labeled RBCs were mixed and transfused. RBC volume was measured in postinfusion peripheral venous blood by quantitating dilution of each population of labeled RBCs.RESULTS: RBC volume measured using either LDB or HDB cells agreed well with FBC volume measured using Cr-51. For the regression of RBC volume by LDB versus RBC volume by Cr-51, correlation = 0.994 and slope = 0.933. For HDB versus Cr-51, correlation = 0.982 and slope = 0.953. RBC volume measured a second time in four subjects with HDB agreed well; mean CV for the differences between HDB and Cr-51 were less than 5 percent.CONCLUSIONS: Using FBCs labeled with two different densities of biotin, RBC volume can be accurately measured simultaneously and repeatedly in the same subject without radiation exposure.
