Correlated Gene Expression and Target Specificity Demonstrate Excitatory Projection Neuron Diversity
Correlated Gene Expression and Target Specificity Demonstrate Excitatory Projection Neuron Diversity
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DOI：
10.1093/cercor/bht243
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发表时间：
2015-02-01
期刊：
影响因子：
3.7
通讯作者：
Lein, Ed S.
中科院分区：
文献类型：
--
作者：
Sorensen, Staci A.;Bernard, Amy;Lein, Ed S.
新皮质包含不同的兴奋性神经元群体，这些神经元按层分离，并可由其特定的皮质和皮质下投射靶点进一步定义。目前的研究描述了一种系统的方法来确定特定的投射神经元类在小鼠初级体感皮层（S1）的分子相关性，使用原位杂交（ISH）数据挖掘，标记基因共定位，并结合逆行标记与ISH层特异性标记基因的组合。首先，我们确定了一个大的基因组与每个皮层的特异性，并显示在这些层的异质模式。使用这些基因作为标记，我们发现了广泛的证据，在层2/3，5和6的基因表达和投影靶特异性的共变，与个别基因标记神经元投射到特定的子集的目标结构。基因表达和靶特异性的组合意味着投射神经元类别的巨大多样性，其类似于或大于GABA能中间神经元。这2个表型模态的协方差表明，这些类别都是离散的和遗传指定的。
The neocortex contains diverse populations of excitatory neurons segregated by layer and further definable by their specific cortical and subcortical projection targets. The current study describes a systematic approach to identify molecular correlates of specific projection neuron classes in mouse primary somatosensory cortex (S1), using a combination of in situ hybridization (ISH) data mining, marker gene colocalization, and combined retrograde labeling with ISH for layer-specific marker genes. First, we identified a large set of genes with specificity for each cortical layer, and that display heterogeneous patterns within those layers. Using these genes as markers, we find extensive evidence for the covariation of gene expression and projection target specificity in layer 2/3, 5, and 6, with individual genes labeling neurons projecting to specific subsets of target structures. The combination of gene expression and target specificity imply a great diversity of projection neuron classes that is similar to or greater than that of GABAergic interneurons. The covariance of these 2 phenotypic modalities suggests that these classes are both discrete and genetically specified.