The effects of nerve growth factor on endothelial cells seeded on different titanium surfaces
The effects of nerve growth factor on endothelial cells seeded on different titanium surfaces
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DOI：
10.1016/j.ijom.2015.06.016
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发表时间：
2015-12-01
影响因子：
2.4
通讯作者：
Gong, P.
中科院分区：
文献类型：
--
作者：
Guang, M.;Yao, Y.;Gong, P.
血管生成对于笔式种植体骨再生和骨整合至关重要。内皮细胞在骨形成早期的血管生成中起重要作用。神经生长因子（NGF）也被报道作为血管生成生长因子。NGF对接种在钛表面上的EC的影响尚不清楚。本研究旨在探讨神经生长因子对笔植入体血管生成的影响。我们使用了两种不同的钛表面。用指定浓度的NGF或血管内皮生长因子（VEGF）处理接种在这些表面上的EC。评估增殖、分化、形态学特征和附着量。采用鸡胚绒毛尿囊膜（CAM）法评价NGF在体内的作用。结果显示，NGF可促进两种钛表面的EC增殖（F-1d = 2.083，P = 0.156; F-3d = 30.857，P = 0.0002; F-5d = 4.440，P = 0.041; F-7 d = 11.065，P = 0.001）。NGF和SLA表面上调NGF、TrkA和p75的mRNA表达（FNGF = 11.941，P = 0.003; F-TrkA = 28.514，P = 0.004; F-p75 = 7.725，P = 0.01）。在体内，NGF处理组的上清液可促进CAM新生血管形成（F = 17.662，P = 0.009）。这项研究表明，神经生长因子可以促进EC增殖，基因表达不同的钛表面，和CAM中的新生血管。这为促进早期牙种植体骨整合提供了新的信息。
Angiogenesis is critical for pen-implant bone regeneration and osseointegration. Endothelial cells (ECs) play an important role in angiogenesis during the early stage of bone formation. Nerve growth factor (NGF) is also reported to function as an angiogenic growth factor. The effects of NGF on ECs seeded on titanium surfaces are unclear. This study was done to investigate the influence of NGF on pen-implant angiogenesis in vitro and in vivo. We used two different titanium surfaces. ECs seeded on these surfaces were treated with indicated concentrations of NGF or vascular endothelial growth factor (VEGF). Proliferation, differentiation, morphological features, and amounts attached were assessed. Chicken embryo chorioallantoic membrane (CAM) was adopted to evaluate the effect of NGF in vivo. The results showed that NGF could promote EC proliferation on both titanium surfaces (F-1d = 2.083, P = 0.156; F-3d = 30.857, P = 0.0002; F-5d = 4.440, P = 0.041; F-7d = 11.065, P = 0.001). NGF and the SLA surface upregulated mRNA of NGF, TrkA, and p75 expression (FNGF = 11.941, P = 0.003; F-TrkA = 28.514, P = 0.004; F-p75 = 7.725, P = 0.01). In vivo, the supernatants of the NGF-treated group could promote neovascularization in CAM (F = 17.662, P = 0.009). This study demonstrated that NGF could enhance EC proliferation, gene expression on different titanium surfaces, and neovascularization in CAM. This provides novel information in relation to the promotion of early dental implant osseointegration.