Intracisternal des-acyl ghrelin inhibits food intake and non-nutrient gastric emptying in conscious rats.
Intracisternal des-acyl ghrelin inhibits food intake and non-nutrient gastric emptying in conscious rats.
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脑池内去酰基生长素释放肽抑制清醒大鼠的食物摄入和非营养性胃排空。
DOI：
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发表时间：
2005
影响因子：
5.4
通讯作者：
M. Doong
中科院分区：
文献类型：
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作者：
Chih‐Yen Chen;Y. Chao;F. Chang;E. Chien;Shou;M. Doong
尽管去酰基生长素释放肽被认为是一种非功能性肽，但研究表明它会减少小鼠的食物摄入和胃排空。然而，尚无研究探讨集中给药的去酰基生长素释放肽对大鼠食物摄入和胃肠道转运的影响。我们研究了脑池内（IC）施用去酰基生长素释放肽对自由喂养和禁食大鼠食物摄入的影响，以及对清醒大鼠胃肠道转运的影响。 IC 注射去酰基生长素释放肽剂量依赖性（0.1 和 1.0 nmol/大鼠）可减少 16 小时禁食大鼠 20 分钟、1 小时和 2 小时累积食物摄入量，但不影响自由进食大鼠，而 IC 注射 O-n-辛酰化（酰化）生长素释放肽（1 nmol/大鼠）可增加进食和禁食大鼠的食物摄入量。与注射盐水的对照相比，IC给药的去酰基生长素释放肽剂量依赖性地抑制木炭半液体胃排空（0.01、0.1、0.3和1.0 nmol/大鼠），但不影响小肠运输的几何中心和运行百分比。然而，IC酰化生长素释放肽增强了胃排空和小肠运输的几何中心，但没有改变小肠运输的运行百分比。研究表明，IC des-acyl ghrelin 可减少食物匮乏大鼠的食物摄入量并抑制胃排空，而不会改变小肠运输。这些结果证实了去酰基生长素释放肽在调节食物摄入和胃排空中的作用。
Although des-acyl ghrelin is thought of as a non-functional peptide, studies show that it decreases food intake and gastric emptying in mice. However, no studies have examined the effects of centrally administered des-acyl ghrelin on food intake and gastrointestinal transit in rats. We investigated the effects of intracisternal (IC) administration of des-acyl ghrelin on food intake in free-feeding and food-deprived rats, as well as on the gastrointestinal transit in conscious rats. IC injection of des-acyl ghrelin dose-dependently (0.1 and 1.0 nmol/rat) decreased 20-min, 1-h and 2-h cumulative food intake in 16-h food-deprived, but not free-feeding rats, while IC administration of O-n-octanoylated (acylated) ghrelin (1 nmol/rat) increased food intake in both fed and fasted rats. IC-administered des-acyl ghrelin dose-dependently inhibited charcoal semi-liquid gastric emptying (0.01, 0.1, 0.3 and 1.0 nmol/rat) compared to saline-injected controls, but did not affect the geometric center and running percentage of small intestinal transit. However, IC acylated ghrelin enhanced gastric emptying and geometric center of small intestinal transit, but did not change running percentage of small intestinal transit. The studies suggest that IC des-acyl ghrelin decreases food intake in food-deprived rats and inhibits gastric emptying without altering small intestinal transit. These results establish the role of des-acyl ghrelin in regulating food intake as well as gastric emptying.