Primary cultures from fetal rat brain incorporate [3H]-isoleucine and [3H]-valine into immunoprecipitable angiotensin II.
Primary cultures from fetal rat brain incorporate [3H]-isoleucine and [3H]-valine into immunoprecipitable angiotensin II.
复制标题
来自胎鼠脑的原代培养物将[3H]-异亮氨酸和[3H]-缬氨酸掺入免疫沉淀血管紧张素II中。
DOI：
10.1159/000123438
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发表时间：
1983
影响因子：
4.1
通讯作者：
Gerndt,JS
中科院分区：
文献类型：
--
作者：
Raizada,MK;Phillips,MI;Gerndt,JS
胎鼠脑原代培养神经元内有血管紧张素II免疫反应。为了研究这种免疫反应的来源，原代培养细胞与[~3H]-Valine或[~3H]-异亮氨酸孵育。观察到放射性与免疫共沉淀血管紧张素II的时间依赖性掺入。用转化酶抑制剂卡托普利孵育培养物，可显著抑制[~3H]-valine掺入免疫反应性[~3H-血管紧张素-II]。这些结果表明，胎鼠脑原代培养可合成血管紧张素样免疫反应，支持血管紧张素-II可能是在胎鼠脑内合成的观点。
Primary cultures from fetal rat brain contain angiotensin II immunoreactivity in neurons. To study the origin of this immunoreactivity, primary cultures were incubated with [3H]-valine or [3H]-isoleucine. A time-dependent incorporation of radioactivity into immunoprecipitable angiotensin II was observed. Incubation of cultures with Captopril, an inhibitor of the converting enzyme, resulted in a significant inhibition of [3H]-valine incorporation into immunoreactive [3H-angiotensin II. These results demonstrate that primary cultures from fetal rat brain can synthesize an angiotensin II-like immunoreactivity and support a concept that angiotensin II may be synthesized in fetal rat brain.