Development of the Liver- and Lobe-Selective Nonviral Gene Transfer Following the Instillation of Naked Plasmid DNA Using Catheter on the Liver Surface in Mice
Development of the Liver- and Lobe-Selective Nonviral Gene Transfer Following the Instillation of Naked Plasmid DNA Using Catheter on the Liver Surface in Mice
复制标题
使用导管将裸质粒 DNA 滴注到小鼠肝脏表面后，开发肝脏和叶选择性非病毒基因转移
DOI：
10.1023/a:1022204127659
复制
发表时间：
2003
影响因子：
3.7
通讯作者：
J. Nakamura
中科院分区：
文献类型：
--
作者：
R. Hirayama;S. Kawakami;K. Nishida;M. Nakashima;H. Sasaki;T. Sakaeda;J. Nakamura
目的。本研究在小鼠肝脏表面使用导管滴注裸质粒DNA（pDNA）后进行了肝脏和肝叶选择性非病毒基因转移。方法。将聚醚尼龙导管通过腹壁插入腹腔内，并保留在肝脏右叶和左内叶的表面。结果：在肝表面滴注pDNA后6 h，在所应用的肝叶中产生的荧光素酶水平显著高于在其它肝叶和其它组织中产生的荧光素酶水平，并且范围从在其它肝叶中高约5倍到在其它组织中高20-30倍。在2 ~ 24 h或15 ~ 60 μl的剂量下，pDNA滴入小鼠肝表面后，肝叶的基因表达量均显著高于其它肝叶和其它组织。结论：我们证实了裸pDNA经导管滴入小鼠肝表面后的肝和肝叶选择性基因转染。
Purpose.The present study has undertaken the liver- and lobe-selective nonviral gene transfer following the instillation of naked plasmid DNA (pDNA) using catheter on the liver surface in mice.Methods.The polyethernylon catheter was inserted intraperitoneally through the abdominal wall and was retained on the surface of the liver right and left medial lobes. pDNA was administered through the catheter to the liver right and left medial lobes.Results.The luciferase levels produced in the applied liver lobes at 6 h after liver surface instillation of pDNA were significantly higher than those produced in other liver lobes and other tissues assayed, and ranged from approximately 5 folds higher in other lobes to 20-30 folds higher in other tissues. Following liver surface instillation of pDNA at a time from 2 to 24 h or at a volume from 15 to 60 μl, the gene expressions of the applied liver lobes were always significantly higher than those of other liver lobes and other tissues.Conclusion.We have demonstrated the liver- and lobe-selective gene transfection following the instillation of naked pDNA using catheter on the liver surface in mice.