Engineering growing tissues
Engineering growing tissues
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DOI：
10.1073/pnas.192291499
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发表时间：
2002-09-17
影响因子：
11.1
通讯作者：
Mooney, DJ
中科院分区：
文献类型：
--
作者：
Alsberg, E;Anderson, KW;Mooney, DJ
再生或工程新的组织和器官可能有一天允许常规替换丢失或失败的组织和器官。然而，这些工程组织不仅必须生长以填补缺陷并与宿主组织整合，而且通常还必须随着身体需求的变化而生长。我们假设能够随时间生长的组织可以通过向细胞移植所用生物材料的细胞提供生长刺激信号来工程化。在这项研究中，软骨细胞和成骨细胞共移植到水凝胶上，该水凝胶用含有RGD的肽序列修饰以促进细胞增殖。新骨组织形成，通过软骨内骨化以类似于正常长骨生长的方式生长。移植的细胞组织成在形态和功能上类似于生长板的结构。这些工程组织可以用于治疗生长板的疾病和损伤，测试实验药物对生长板功能和发育的影响，以及研究长骨生长的生物学。此外，这种促进工程化组织生长的概念可以在通过移植少量前体细胞的方式工程化多种组织类型中找到很大的实用性。
Regenerating or engineering new tissues and organs may one day allow routine replacement of lost or failing tissues and organs. However, these engineered tissues must not only grow to fill a defect and integrate with the host tissue, but often they must also grow in concert with the changing needs of the body over time. We hypothesized that tissues capable of growing with time could be engineered by supplying growth stimulus signals to cells from the biomaterial used for cell transplantation. In this study, chondrocytes and osteoblasts were cotransplanted on hydrogels modified with an RGD-containing peptide sequence to promote cell multiplication. New bone tissue was formed that grew in mass and cellularity by endochondral ossification in a manner similar to normal long-bone growth. Transplanted cells organized into structures that morphologically and functionally resembled growth plates. These engineered tissues could find utility in treating diseases and injuries of the growth plate, testing the effect of experimental drugs on growth-plate function and development, and investigating the biology of long-bone growth. Furthermore, this concept of promoting the growth of engineered tissues could find great utility in engineering numerous tissue types by way of the transplantation of a small number of precursor cells.