Biochemical characterization of SARS-CoV-2 nucleocapsid protein
Biochemical characterization of SARS-CoV-2 nucleocapsid protein
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SARS-CoV-2 核衣壳蛋白的生化特征
DOI：
10.1016/j.bbrc.2020.04.136
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发表时间：
2020-06-30
影响因子：
3.1
通讯作者：
Jin, Tengchuan
中科院分区：
文献类型：
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作者：
Zeng, Weihong;Liu, Guangfeng;Jin, Tengchuan
核衣壳(N)蛋白是冠状病毒的重要抗原，参与RNA包装和病毒颗粒释放。在本研究中，我们表达了SARS-CoV-2的N蛋白，并对其生化特性进行了鉴定。静态光散射、大小排斥层析和小角X射线散射(SAXS)分析表明，纯化的N蛋白主要为二聚体。圆二色谱表明，它在室温下有很高比例的无序区，而在55℃时结构最好，这表明它的结构动力学。荧光偏振分析显示它具有非特异性的核酸结合能力，这引起了人们对其作为诊断标志物的关注。免疫印迹试验证实新冠肺炎感染者血清中存在针对N抗原的Ig A、Ig M和Ig G抗体，证明了该抗原在宿主免疫和诊断中的重要性。(C)Elsevier Inc.出版的2020年。
The nucleocapsid (N) protein is an important antigen for coronavirus, which participate in RNA package and virus particle release. In this study, we expressed the N protein of SARS-CoV-2 and characterized its biochemical properties. Static light scattering, size exclusive chromatography, and small-angle X-ray scattering (SAXS) showed that the purified N protein is largely a dimer in solution. CD spectra showed that it has a high percentage of disordered region at room temperature while it was best structured at 55 degrees C, suggesting its structural dynamics. Fluorescence polarization assay showed it has non-specific nucleic acid binding capability, which raised a concern in using it as a diagnostic marker. Immunoblot assays confirmed the presence of IgA, IgM and IgG antibodies against N antigen in COVID-19 infection patients' sera, proving the importance of this antigen in host immunity and diagnostics. (C) 2020 Published by Elsevier Inc.