Isolation and characterization of the lipopolysaccharide of Chromatium vinosum.
Isolation and characterization of the lipopolysaccharide of Chromatium vinosum.
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Chromatium vinosum 脂多糖的分离和表征。
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
I. Fromme
中科院分区：
文献类型：
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作者：
R. E. Hurlbert;J. Weckesser;H. Mayer;I. Fromme
采用苯酚/水抽提法从花色草中分离得到脂多糖。该脂多糖仅存在于苯酚相中，在100℃、10%的醋酸中水解3-4小时，可将其裂解为糖部分和类脂A部分，其中含有中性糖3-O-甲基-D-核糖、D-核糖、L-阿拉伯糖、甘露糖和葡萄糖，以及少量的D-鼠李糖、D-甘油-D-甘露-庚糖、喹诺夫胺和2-酮-3-脱氧葡萄糖。未检测到L-甘油-D-甘露七糖。葡萄球菌脂多糖中的2-酮-3-脱氧辛酸键比大肠杆菌的2-酮-3-脱氧辛酸键更耐酸水解。脂A组分含有氨基葡萄糖、甘露糖和脂多糖的脂肪酸。主要的脂肪酸是β-羟基肉豆酸，少量的月桂酸和棕榈酸以及14碳单不饱和脂肪酸也存在。文氏囊藻脂多糖的磷含量约为0.1%。用抗热灭活细胞的抗血清凝集经碱处理的醋酸杆菌脂多糖致敏的红细胞。未经处理或加热处理的脂多糖不会使红细胞致敏。该脂多糖对小鼠的致死毒性约为马流产沙门氏菌的十分之一。
Lipolysaccharide was isolated from Chromatium vinosum by phenol/water extraction. The lipopolysaccharide is found exclusively in the phenol phase and can be cleaved into a sugar moiety and a lipid A fraction by hydrolysis in 10% acetic acid at 100 degrees C for 3-4 h. The sugar moiety contains the neutral sugars 3-O-methyl-D-ribose, D-ribose, L-arabinose, mannosamine and glucose, and smaller quantities of D-rhamnose, D-glycero-D-manno-heptose (tentatively identified), quinovosamine and 2-keto-3-deoxyoctonate. L-glycero-D-manno-heptose was not detected. The 2-keto-3-deoxyoctonate linkage in C. vinosum lipopolysaccharide is more resistant to acid hydrolysis than that of Escherichia coli. The lipid A fraction contains glucosamine, mannose and the fatty acids of the lipopolysaccharide. The major fatty acid is beta-hydroxymyristic acid, with smaller amounts of lauric and palmitic acids as well as 14-carbon mono-unsaturated fatty acid, also being present. The phosphorus content of the C. vinosum lipopolysaccharide was found to be approximately 0.1%. Erythrocytes sensitized with alkali-treated C. vinosum lipopolysaccharide were agglutinated by antisera prepared against heat-killed cells. Untreated or heat-treated lipopolysaccharide did not sensitize erythrocytes. The lethal toxicity to mice of the C. vinosum lipopolysaccharide is about one-tenth as that from Salmonella abortus equi.