Expression, purification, crystallization and preliminary X-ray diffraction analysis of mitochondrial thioredoxin Trx3 from Saccharomyces cerevisiae.
Expression, purification, crystallization and preliminary X-ray diffraction analysis of mitochondrial thioredoxin Trx3 from Saccharomyces cerevisiae.
复制标题
酿酒酵母线粒体硫氧还蛋白 Trx3 的表达、纯化、结晶和初步 X 射线衍射分析。
DOI：
10.1107/s1744309106041467
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Cong
中科院分区：
文献类型：
--
作者：
R. Bao;Yuxing Chen;Yaru Zhang;Cong
在酿酒酵母中有三种硫氧还蛋白亚型：两种胞质/核硫氧还蛋白，Trx 1和Trx 2，以及一种线粒体硫氧还蛋白，Trx 3。在目前的工作中，S.将在大肠杆菌中过表达的酿酒酵母Trx 3纯化并结晶。通过悬滴气相扩散法获得Trx 3晶体。从单晶收集衍射至2.0 A分辨率的数据集。晶体属于空间群P3（1），晶胞参数a = B = 49.57，c = 94.55，α = β = 90，γ = 120度。假设不对称单元包含Trx 3的两个亚基，V（M）值为2.62 A（3）Da（-1），溶剂含量为53%。
There are three thioredoxin isoforms in the yeast Saccharomyces cerevisiae: two cytosolic/nuclear thioredoxins, Trx1 and Trx2, and one mitochondrial thioredoxin, Trx3. In the present work, S. cerevisiae Trx3 overexpressed in Escherichia coli was purified and crystallized. The Trx3 crystals were obtained by the hanging-drop vapour-diffusion method. A data set diffracting to 2.0 A resolution was collected from a single crystal. The crystal belongs to space group P3(1), with unit-cell parameters a = b = 49.57, c = 94.55 A, alpha = beta = 90, gamma = 120 degrees. The asymmetric unit is assumed to contain two subunits of Trx3, with a V(M) value of 2.62 A(3) Da(-1) and a solvent content of 53%.