An array of early differentiating cones precedes the emergence of the photoreceptor mosaic in the fetal monkey retina.
An array of early differentiating cones precedes the emergence of the photoreceptor mosaic in the fetal monkey retina.
复制标题
在胎猴视网膜中光感受器镶嵌体出现之前，出现了一系列早期分化的视锥细胞。
DOI：
10.1073/pnas.91.14.6534
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发表时间：
1994
影响因子：
11.1
通讯作者：
Rakic,P
中科院分区：
文献类型：
--
作者：
Wikler,KC;Rakic,P
我们以前已经证明，大约10%的视锥细胞在胎猴视网膜早熟表达红/绿色视蛋白。这些数据表明，锥细胞的一个子集分化之前，他们的新生锥邻居的可能性。为了进一步评估这种早期视锥细胞分化在胎猴视网膜，我们使用的单克隆抗体被证明是感光细胞表型和突触发生的重要发育标志物（XAP-1，特异性感光膜; SV 2，特异性突触囊泡蛋白）。虽然这两种抗体识别功能不同的抗原，我们的分析表明，都确定一个子集的早熟免疫反应锥。此外，XAP-1和SV 2阳性视锥细胞以与胎猴视网膜未成熟区域中早熟的红/绿敏感视锥细胞相同的模式分布。这些结果支持的假设，灵长类动物视网膜具有空间组织的protomap，可能会导致出现的光感受器马赛克，并触发形成的颜色特异性途径，包括水平，双极，和视网膜神经节细胞。
We previously have demonstrated that approximately 10% of cones in the fetal monkey retina precociously express the red/green opsin. These data suggested the possibility that a subset of cones differentiates prior to their nascent cone neighbors. To further assess this early cone differentiation in the fetal monkey retina, we used monoclonal antibodies proven to be important developmental markers of photoreceptor phenotypes and synaptogenesis (XAP-1, specific to photoreceptor membranes; SV2, specific to synaptic vesicle protein). Although these two antibodies recognize functionally distinct antigens, our analyses revealed that both identify a subset of precociously immunoreactive cones. Further, XAP-1- and SV2-positive cones are distributed in the same pattern as precocious red/green-sensitive cones in immature regions of the fetal monkey retina. These results support the hypothesis that the primate retina possesses a spatially organized protomap that may induce the emergence of the photoreceptor mosaic and trigger the formation of color-specific pathways that include horizontal, bipolar, and retinal ganglion cells.