Genetic variation in the timing of first cleavage in mice: effect of maternal genotype.
Genetic variation in the timing of first cleavage in mice: effect of maternal genotype.
复制标题
小鼠第一次卵裂时间的遗传变异：母体基因型的影响。
DOI：
10.1095/biolreprod23.2.369
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发表时间：
1980
影响因子：
3.6
通讯作者：
W. Whitten
中科院分区：
文献类型：
--
作者：
J. Shire;W. Whitten
将体内受精的11种基因型雌性小鼠的卵子在限定培养基中进行体外培养。对于BALB/cBy母体，BALB/cWt雄性受精卵第一次卵裂的中位时间为24.8小时，对于C57 BL/6 By或C57 X BALB Fl母体，第一次卵裂的中位时间约为22小时。来自7个重组近交系的雌性的卵的第一卵裂的中位时间范围从CXBI中的20.5小时到CXBJ中的26.8小时。第一次卵裂的时间与雌雄同体后代发生的风险没有明显的关系，这在重组近交系之间也是不同的。当与从不产生雌雄同体后代的SJL X SWR Fl雄性交配时，BALB X C57 Fl、CXBH和SJL X SWR Fl雌性产生在不同时间裂解的卵，但在所有情况下都早于由BALB/cWt雄性受精的相同基因型的卵。来自CXBJ雌性的受精卵通常成功地经历了第一次卵裂，但在培养中未能进一步发育。几乎所有来自其他基因型雌性的卵都成功形成囊胚。已经完成第一次卵裂的卵如果由BALB/cWt雄性受精，则形成囊胚的可能性比由其他雄性受精的可能性小。
Eggs from female mice of eleven genotypes that had been fertilized in vivo were cultured in vitro in defined medium. The median time of first cleavage of eggs fertilized by BALB/cWt males was 24.8 h with BALB/cBy mothers and about 22 h with C57BL/6By or C57 X BALB Fl mothers. Eggs from females of seven recombinant-inbred lines had median times of first cleavage that ranged from 20.5 h in CXBI to 26.8 h in CXBJ. The timing of first cleavage bore no apparent relationship to the risk of occurrence of hermaphrodite offspring, which also differed between the recombinant- inbred lines. When mated to SJL X SWR Fl males, which never produce hermaphrodite off- spring, BALB X C57 Fl, CXBH, and SJL X SWR Fl females produced eggs that cleaved at differ- ent times, but in all cases earlier than eggs of the same genotype fertilized by BALB/cWt males. Fertilized eggs from CXBJ females often underwent first cleavage successfully but failed to develop further in culture. Almost all eggs from females of the other genotypes formed blastocysts successfully. Eggs that had completed first cleavage were somewhat less likely to form blastocysts if they had been fertilized by a BALB/cWt male than if they had been fertilized by some other male.