In Vitro and In Vivo Differentiation of Human Embryonic Stem Cells Into Retina-Like Organs and Comparison With That From Mouse Pluripotent Epiblast Stem Cells
In Vitro and In Vivo Differentiation of Human Embryonic Stem Cells Into Retina-Like Organs and Comparison With That From Mouse Pluripotent Epiblast Stem Cells
复制标题
DOI：
10.1002/dvdy.22008
复制
发表时间：
2009-09-01
影响因子：
2.5
通讯作者：
Kunisada, Takahiro
中科院分区：
文献类型：
--
作者：
Aoki, Hitomi;Hara, Akira;Kunisada, Takahiro
正确地诱导多能hESCs向高纯度的特定谱系分化是再生细胞治疗非常需要的。我们对hESCs进行体外分化的第一次努力导致了有限的眼部组织结构重现。当未分化的hESCs在体内植入眼组织时，在这种情况下，植入玻璃体腔，产生三维视网膜样结构，使人联想到视神经囊泡内陷。免疫组织化学分析证实存在神经视网膜样细胞层和视网膜色素上皮样细胞层，可能相当于发育中的E12.5小鼠视网膜。此外，小鼠外成细胞来源的干细胞也能在体内产生视网膜样角度结构，据报道，这些干细胞与hESCs具有某些共同特征，但与小鼠ESCs不同。hesc衍生的视网膜样结构为视网膜疾病的治疗提供了新的可能性，也为研究人类早期眼睛发育提供了新的实验系统。科学通报，2009(4):344 - 344。(C) 2009 Wiley-Liss, Inc。
Correctly inducing the differentiation of pluripotent hESCs to a specific lineage with high purity is highly desirable for regenerative cell therapy. Our first effort to perform in vitro differentiation of hESCs resulted in a limited recapitulation of the ocular tissue structures. When undifferentiated hESCs were placed in vivo into the ocular tissue, in this case into the vitreous cavity, 3-dimensional retina-like structures reminiscent of the invagination of the optic vesicle were generated. Immunohistochemical analysis confirmed the presence of both a neural retina-like cell layer and a retinal pigmented epithelium-like cell layer, possibly equivalent to the developing E12.5 mouse retina. Furthermore, mouse epiblast-derived stem cells, which are reported to share some characteristics with hESCs, but not with mouse ESCs, also generated retinal anlage-like structures in vivo. hESC-derived retina-like structures present a novel therapeutic possibility for retinal diseases and also provide a novel experimental system to study early human eye development. Developmental Dynamics 238:2266-2279, 2009. (C) 2009 Wiley-Liss, Inc.