Increased oxygen exposure alters collagen expression and tissue architecture during ligature-induced periodontitis.
Increased oxygen exposure alters collagen expression and tissue architecture during ligature-induced periodontitis.
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DOI：
10.1111/jre.12408
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发表时间：
2017-06
影响因子：
3.5
通讯作者：
Engeland CG
中科院分区：
文献类型：
--
作者：
Gajendrareddy PK;Junges R;Cygan G;Zhao Y;Marucha PT;Engeland CG
本研究的目的是评估增加氧的可用性对基因表达和胶原蛋白沉积/成熟的牙周疾病后的影响。将雄性Wistar大鼠的臼齿周围结扎以诱导牙周病，并对一组动物进行高压氧（HBO）治疗，每天两次，每次两小时。在第15天和第28天，测定COL 1A 1、TGF-β1和ALP的组织基因表达;用苦味酸天狼星红对其他组织学样本进行染色，以评价胶原沉积、成熟和厚度的水平。在接受高压氧治疗的动物中，I型胶原蛋白表达更高，胶原蛋白沉积、成熟和厚度更强。HBO治疗大鼠在第28天TGF-β1和ALP的mRNA水平降低，表明氧治疗后软组织和骨重建的解决速度更快。两组之间未观察到炎症差异。总之，细胞外基质（ECM）再生更快，在HBO治疗组证明了更高的胶原蛋白的表达，沉积和成熟。
The aim of this study was to evaluate the effects of increased oxygen availability on gene expression and on collagen deposition/maturation in the periodontium following disease. Male Wistar rats had ligatures placed around their molars to induce periodontal disease, and a subset of animals underwent hyperbaric oxygen (HBO) treatment for two hours twice per day. At 15 and 28 days, tissue gene expression of COL1A1, TGF-β1 and ALP was determined; other histological samples were stained with Picrosirius red to evaluate levels of collagen deposition, maturation and thickness. In animals that underwent HBO treatment, type I collagen expression was higher and collagen deposition, maturation and thickness were more robust. Reduced mRNA levels of TGF-β1 and ALP in HBO-treated rats on day 28 suggested that a quicker resolution in both soft tissue and bone remodeling occurred following oxygen treatment. No differences in inflammation were observed between groups. In conclusion, the extra-cellular matrix (ECM) regenerated more quickly in the HBO-treated group as evidenced by higher collagen expression, deposition and maturation.