Bifurcation in Blood Oscillatory Rhythms for Patients with Ischemic Stroke: A Small Scale Clinical Trial using Laser Doppler Flowmetry and Computational Modeling of Vasomotion.
Bifurcation in Blood Oscillatory Rhythms for Patients with Ischemic Stroke: A Small Scale Clinical Trial using Laser Doppler Flowmetry and Computational Modeling of Vasomotion.
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DOI：
10.3389/fphys.2017.00160
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发表时间：
2017
影响因子：
4
通讯作者：
Rafailov E
中科院分区：
文献类型：
--
作者：
Goltsov A;Anisimova AV;Zakharkina M;Krupatkin AI;Sidorov VV;Sokolovski SG;Rafailov E
我们描述了应用激光多普勒血流仪（LDF）信号的频谱分析，以调查急性缺血性卒中（AIS）后和脑血管功能不全患者的脑血管血流动力学。在AIS发病后3 ~ 7天对患者的右、左眶上区前额进行LDF检查。LDF信号分析显示AIS患者的微血管灌注低于脑血管功能不全患者。作为LDF信号的小波分析的结果，我们获得了激活的血管运动的频率范围内的肌源性振荡的0.1 Hz和主要营养制度的AIS患者的全身溶栓治疗后的微循环。在显著卒中大小、肌源性活动和营养模式微血流动力学降低的情况下，在某些情况下，显示非营养模式和/或小静脉淤滞。LDF信号的小波分析也显示了AIS患者中风影响和未受影响半球中获得的LDF信号的小波谱的不对称性。所观察到的不对称性的机制进行了分析，在Arciero和Secomb开发的血管运动的计算建模。我们应用这个模型来描述血管平滑肌细胞中同步钙振荡引起的肌源性振荡所强迫的小动脉直径的弛豫振荡。计算表明，在低频范围（0.01 Hz）的血管运动频谱是由肌源性振荡（0.1 Hz），与实验观察的LDF频谱中的半球间的变化的调制。
We describe application of spectral analysis of laser Doppler flowmetry (LDF) signals to investigation of cerebrovascular haemodynamics in patients with post-acute ischemic stroke (AIS) and cerebrovascular insufficiency. LDF was performed from 3 to 7 days after the onset of AIS on forehead in the right and left supraorbital regions in patients. Analysis of LDF signals showed that perfusion in the microvasculature in AIS patients was lower than that in patients with cerebrovascular insufficiency. As a result of wavelet analysis of the LDF signals we obtained activation of the vasomotion in the frequency range of myogenic oscillation of 0.1 Hz and predominantly nutritive regime microcirculation after systemic thrombolytic therapy of the AIS patients. In case of significant stroke size, myogenic activity, and nutritive pattern microhaemodynamics were reduced, in some cases non-nutritive pattern and/or venular stasis was revealed. Wavelet analysis of the LDF signals also showed asymmetry in wavelet spectra of the LDF signals obtained in stroke-affected and unaffected hemispheres in the AIS patients. A mechanism underlying the observed asymmetry was analyzed by computational modeling of vasomotion developed in Arciero and Secomb. We applied this model to describe relaxation oscillation of arteriole diameter which is forced by myogenic oscillation induced by synchronous calcium oscillation in vascular smooth muscle cells. Calculation showed that vasomotion frequency spectrum at the low-frequency range (0.01 Hz) is reciprocally modulated by myogenic oscillation (0.1 Hz) that correlates with experimental observation of inter-hemispheric variation in the LDF spectrum.