Microarray Analysis of Host Immune Responses to Marek's Disease Virus Infection in Vaccinated Chickens
Microarray Analysis of Host Immune Responses to Marek's Disease Virus Infection in Vaccinated Chickens
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DOI：
10.1292/jvms.71.603
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发表时间：
2009-05-01
影响因子：
1.2
通讯作者：
Ohashi, Kazuhiko
中科院分区：
文献类型：
--
作者：
Kano, Rika;Konnai, Satoru;Ohashi, Kazuhiko
马立克氏病（MD）是由马立克氏病（MDV）引起的一种重要的商业鸡疾病。尽管无毒力的MDV毒株已被用于疫苗接种以预防MD爆发，但疫苗的保护机制尚未阐明。在本研究中，在感染后 7 天和 21 天 (dpi)，使用微阵列智慧对接种和未接种疫苗的 MDV 感染鸡进行了全面的转录分析。数据表明，与未接种疫苗的鸡相比，在感染潜伏期，接种疫苗的鸡中 T 细胞受体 (TCR) 1 相关基因的表达上调。一致地，这种诱导通过定量PCR得到证实。流式细胞术分析显示大多数TCR1(+)细胞明亮地表达CD8α链。与未接种疫苗的鸡相比，在 21 dpi 时接种疫苗的鸡中该亚群的数量明显增加，尽管它不是鸡脾脏中的主要群体。与未接种疫苗的对照鸡相比，无论有或没有攻击，CD8α（高）TCR2（+）细胞（鸡CD8α（高）细胞的主要亚群）的数量在接种疫苗的鸡中有所增加。这些数据表明，疫苗接种可以诱导CD8α（高）TCR1（+）和CD8α（高）TCR2（+）细胞。 CD8α（高）TCR1（+）细胞也可能通过疫苗接种而启动，并在感染潜伏期通过MDV强毒株的攻击而被特异性诱导。因此，CD8α（高）TCR1（+）细胞群可能是疫苗株CV1988诱导的保护机制中涉及的关键因素之一。
Marek's disease (MD) is a commercially important disease of chickens caused by MD virus (MDV). Although avirulent MDV strains have been used for vaccination to prevent MD outbreaks, the protective mechanism of the vaccine has not been elucidated. In this Study, a comprehensive transcriptional analysis using microarray wits conducted in MDV-infected chickens with and without vaccination at 7 and 21 days post-infection (dpi). The data Suggested that the expression of T cell receptor (TCR) 1-related genes was up-regulated in vaccinated-challenged compared to unvaccinated-challenged chickens during the latent phase of infection. Consistently, this induction was confirmed by quantitative PCR. Flow cytometric analysis revealed that most of TCR1(+) cells expressed CD8 alpha chain brightly. The number of this subpopulation was significantly and specifically increased in vaccinated-challenged chickens at 21 dpi compared to univaccinated-challenged chickens, though it was not the major Population in spleen of chickens. The number of CD8 alpha(high) TCR2(+) cells, the major subpopulation of chicken CD8 alpha(high) cells, was increased in vaccinated chickens with or without challenge compared to univaccinated control chickens. These data suggested that both CD8 alpha(high) TCR1(+) and CD8 alpha(high) TCR2(+) cells could be induced by the vaccination. It is also possible that CD8 alpha(high) TCR1(+) cells might be primed by the vaccination and specifically induced by the challenge with virulent strain of MDV during the latent phase of infection. Thus, CD8 alpha(high) TCR1(+) cell Population is probably one of the key factors involved in the protective mechanism induced by a vaccine strain, CV1988.