Molecular genetic studies of Anopheles stephensi in Pakistan
Molecular genetic studies of Anopheles stephensi in Pakistan
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DOI：
10.1111/j.1365-2915.2007.00691.x
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发表时间：
2007-09-01
影响因子：
1.9
通讯作者：
Donnelly, M. J.
中科院分区：
文献类型：
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作者：
Ali, N.;Hume, J. C. C.;Donnelly, M. J.
斯氏按蚊Anopheles stephensi Liston s.l.(双翅目：蚊科)是巴基斯坦、印度、伊朗和阿富汗疟疾的主要媒介之一。在其活动范围的一部分，该物种的相对丰度和绝对丰度都有所增加，这被认为是对广泛灌溉导致的人类中介环境变化的反应。我们试图基于从旁遮普省灌区内的两个村庄(149/6R和111/6R)以及巴基斯坦西北边境省(NWFP)灌溉方案外的一个村庄(Azakhel)获得的三个样本，使用7个微卫星座位和682个线粒体CO1基因碱基对，来检测这种种群不稳定的分子遗传特征。微卫星座位在群体内表现出较高的遗传多样性(平均每个座位的等位基因数为10.71~11.57，平均杂合度为0.703~0.733)。在21个测试中，只观察到两个微卫星座位偏离了Hardy-Weinberg的预期。没有观察到群体间的遗传分化，在任何群体对或任何一个标记系统中，平均成对F-ST值都没有显着高于零。线粒体和微卫星基因座的种群扩张测试都没有定论。
Anopheles stephensi Liston s.l. (Diptera: Culicidae) is one of the major vectors of malaria in Pakistan, India, Iran and Afghanistan. In parts of its range this species has shown increases in both relative and absolute abundance in what is hypothesized to be a response to human-mediated environmental change resulting from extensive irrigation. We attempted to detect the molecular genetic signatures of this population instability based on three samples obtained from two villages (149/6R and 111/6R) within an irrigation zone in Punjab Province and from one village (Azakhel) outside the irrigation scheme in Northwest Frontier Province (NWFP), Pakistan, using seven microsatellite loci and 682 basepairs of the mitochondrial CO1 gene. For microsatellite loci, high levels of genetic diversity were observed within populations (mean alleles per locus 10.71-11.57; mean heterozygosity 0.703-0.733). Deviation from Hardy-Weinberg expectations was observed for only two microsatellite loci in 21 tests. No genetic differentiation was observed between populations and average pairwise F-ST values did not differ significantly from zero for any population pair or either marker system. Tests of population expansion for both mitochondrial and microsatellite loci were inconclusive.