The varicella-zoster virus induces apoptosis in vitro in subpopulations of primary human peripheral blood mononuclear cells
The varicella-zoster virus induces apoptosis in vitro in subpopulations of primary human peripheral blood mononuclear cells
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DOI：
10.1016/s1286-4579(03)00177-1
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发表时间：
2003-08-01
影响因子：
5.8
通讯作者：
Wolff, MH
中科院分区：
文献类型：
--
作者：
König, A;Hömme, C;Wolff, MH
水痘-带状疱疹病毒（Varicella-zostervirus，VZV）是疱疹病毒科（Herpesviridae）α-疱疹病毒亚科（alpha-Herpesvirinae）的一种，是人类特有的致病性病毒。水痘是VZV初次感染的结果。在病毒血症阶段，VZV感染外周血单核细胞（PBMC）并扩散到外周。在皮肤细胞中，它会引起典型的病变。其他α-疱疹病毒已在不同细胞类型中证实了细胞凋亡。VZV感染的T淋巴细胞，B淋巴细胞和单核细胞，分别在本体外研究中，通过流式细胞术，免疫荧光和电子显微镜检查。所有受感染的细胞类型均表现出凋亡的迹象：DNA含量降低、DNA片段化、膜完整性丧失和核形态改变。观察到的结果表明，VZV在体内感染过程中可以诱导PBMC亚群的凋亡。(C)2003年，Elsevier SAS科学与医学版。All rights reserved.
Varicella-zoster virus (VZV), a member of Herpesviridae, subfamily alpha-Herpesvirinae, is pathogenic exclusively in the human. Chickenpox is the result of primary infection of VZV. During the viremic stage, VZV infects peripheral blood mononuclear cells (PBMC) and spreads to the periphery. In skin cells it causes typical lesions. Apoptosis has been demonstrated in different cell types by other a-herpesviruses. VZV-infected T lymphocytes, B lymphocytes, and monocytes, respectively, were examined in this in vitro study by flow cytometry, immunofluorescence and electron microscopy. All infected cell types showed signs of apoptosis: a lower DNA content, DNA fragmentation, loss of membrane integrity, and an altered nuclear morphology. The results observed led to the suggestion that VZV can induce apoptosis during infection in vivo in the PBMC subpopulations. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved.