Regulation of mating and filamentation genes by two distinct Ste12 complexes in Saccharomyces cerevisiae
Regulation of mating and filamentation genes by two distinct Ste12 complexes in Saccharomyces cerevisiae
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DOI：
10.1128/mcb.02053-05
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发表时间：
2006-07-01
影响因子：
5.3
通讯作者：
Liu, Haoping
中科院分区：
文献类型：
--
作者：
Chou, Song;Lane, Shelley;Liu, Haoping
酿酒酵母转录因子Ste 12控制着交配和交配两种不同的发育程序。丝裂原活化蛋白激酶Fus 3和Kss 1通过两种Ste 12抑制剂Dig 1和Dig 2调节Ste 12活性。交配基因由Ste 12通过Ste 12结合位点（信息素反应元件[PREs]）调节，而交配基因被推测由与Tec 1结合位点（TCS）相邻的PRE上的Ste 12和Tec 1的合作结合调节，称为丝状反应元件（FRE）。然而，大多数表达基因不包含FRE;相反，它们都具有TCS。通过免疫沉淀，我们表明，Ste 12形成两个不同的复合物，Stel 2/Dig 1/Dig 2和Tec 1/Ste 12/Dig 1，在体内和体外。这两种复合物是由Tec 1和Dig 2与Ste 12竞争结合形成的，因为Tec 1可以在体外和体内从Ste 12中竞争Dig 2。在Tec 1/Ste 12/Dig 1复合物中，Tec 1与Ste 12的N末端结合，并通过Ste 12间接与Dig 1结合。Tec 1具有低的基础活性，其转录激活由相关的Ste 12提供，Ste 12处于Dig 1抑制下。成丝基因由Tec 1/Stel 2/Digl复合物结合，而交配基因主要由Ste 12/Dig 1/Dig 2和一些Tec 1/Ste 12/Dig 1占据。我们认为Tec 1将Ste 12与TCS元件连接在表达基因的上游，并将表达基因定义为Ste 12调控基因的一个子集。
The Saccharomyces cerevisiae transcription factor Ste12 controls two distinct developmental programs of mating and filamentation. Ste12 activity is regulated by Fus3 and Kss1 mitogen-activated protein kinases through two Ste12 inhibitors, Dig1 and Dig2. Mating genes are regulated by Ste12 through Ste12 binding sites (pheromone response elements [PREs]), whereas filamentation genes are supposedly regulated by the cooperative binding of Ste12 and Tec1 on a PRE adjacent to a Tec1-binding site (TCS), termed filamentous responsive element (FRE). However, most filamentation genes do not contain an FRE; instead, they all have a TCS. By immunoprecipitation, we show that Ste12 forms two distinct complexes, Stel2/Dig1/Dig2 and Tec1/Ste12/Dig1, both in vivo and in vitro. The two complexes are formed by the competitive binding of Tec1 and Dig2 with Ste12, as Tec1 can compete off Dig2 from Ste12 in vitro and in vivo. In the Tec1/Ste12/Dig1 complex, Tec1 binds to the N terminus of Ste12 and to Dig1 indirectly through Ste12. Tec1 has low basal activity, and its transcriptional activation is provided by the associated Ste12, which is under Dig1 inhibition. Filamentation genes are bound by the Tec1/Stel2/Digl complex, whereas mating genes are occupied by mostly Ste12/Dig1/Dig2 with some Tec1/Ste12/Dig1. We suggest that Tec1 tethers Ste12 to TCS elements upstream of filamentation genes and defines the filamentation genes as a subset of Ste12-regulated genes.