High-performance capillary electrophoretic characterization of different types of oligo- and polysialic acid chains
High-performance capillary electrophoretic characterization of different types of oligo- and polysialic acid chains
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DOI：
10.1006/abio.1998.2701
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发表时间：
1998-07-01
影响因子：
2.9
通讯作者：
Inoue, Y
中科院分区：
文献类型：
--
作者：
Cheng, MC;Lin, SL;Inoue, Y
我们对不同来源的新型寡唾液酸链和聚唾液酸链进行了比较结构分析。 (a) 多聚氨基酸、N-乙酰神经氨酸 (Neu5Ac) 的 α 2-->8-连接均聚物，(b) 虹鳟鱼卵聚唾液酸糖蛋白中存在的 α 2-->8-连接寡聚/聚 Neu5Gc 链，以及 (c) 源自富含 KDN 的糖蛋白的脱氨基神经氨酸 (KDN) 残基的 α 2-->8-连接寡聚物的受控酸水解产物通过高性能毛细管电泳 (HPCE) 分析虹鳟卵黄膜。结果表明，HPCE可以分离三种不同类型的具有相同聚合度的α2→8-连接的低聚唾液酸。 CE 显示，在受控水解和随后的后处理过程中，多聚亚麻酸与弱酸的部分水解产物形成分子内酯。相比之下，存在于海胆蛋冻皮中的 (-->5-O-乙醇酰基-Neu5Gc α 2-->)n（α 2-->5-O-乙醇酰基连接的 N-乙醇酰神经氨酸均聚物 (Neu5Gc)）的内酯化并不像 (-->8Neu5Ac α 2-->)n 那样容易发生。 (C) 1998 年学术出版社。
We have carried out comparative structural analysis of novel oligo- and polysialic acid chains from diverse sources. Controlled acid hydrolysates of (a) colominic acid, alpha 2-->8-linked homopolymer of N-acetylneuraminic acid (Neu5Ac), (b) alpha 2-->8-linked oligo/polyNeu5Gc chains present in rainbow trout egg polysialoglycoprotein, and (c) alpha 2-->8-linked oligomers of deaminoneuraminic acid (KDN) residues of KDN-rich glycoprotein derived from rainbow trout vitelline envelope were analyzed by highperformance capillary electrophoresis (HPCE). The results showed that three different types of alpha 2-->8-linked oligosialic acids having same degree of polymerization can be separated by HPCE. A partial hydrolysate of colominic acid with mild acid was shown by CE to form intramolecular esters during the controlled hydrolysis and the subsequent workup procedure. In contrast, lactonization of (-->5-O-glycolyl-Neu5Gc alpha 2-->)n, alpha 2-->5-O-glycolyl-linked homopolymer of N-glycolylneuraminic acid (Neu5Gc) present in the egg jelly coat of sea urchin, did not take place as readily as in (-->8Neu5Ac alpha 2-->)n. (C) 1998 Academic Press.