Introduction of Artemia nauplii during Chinese perch (Siniperca chuatsi) first feeding: Effects on larvae growth, survival, gene expression and activity of critical digestive enzymes
Introduction of Artemia nauplii during Chinese perch (Siniperca chuatsi) first feeding: Effects on larvae growth, survival, gene expression and activity of critical digestive enzymes
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DOI：
10.1016/j.aquaculture.2023.739619
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发表时间：
2023-05-01
期刊：
影响因子：
4.5
通讯作者：
Tang，Shulin
中科院分区：
文献类型：
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作者：
Peng，Di;Liang，Xu-Fang;Tang，Shulin
研究了不同投喂方式对中华鲟仔鱼生长、存活、食欲、消化、吸收和生长等基因表达及消化酶活性的影响。在3天后孵化（DPH）的仔鱼中，以3至15个DPH的猎物鱼苗（新孵化的斑马鱼Danio rerio）/卤虫（Artemianaupli）喂养如下不同时间段：（A）仅猎物鱼苗;（B）3-5个DPH卤虫/6-15个DPH猎物鱼苗;（C）3-7个DPH卤虫/8-15个DPH猎物鱼苗;（D）卤虫和猎物鱼苗的组合。最终的性能参数（全长，体重，成活率），基因表达和消化酶的处理之间进行了测量和比较，在15个DPH。A组（53.67 ± 1.76%）和D组（61.22 ± 1.18%）的比生长率显著高于其他各组（P< 0.05）。不同饲养方式对脑组织npy和agrp表达无明显影响。以卤虫7 DPH为饵料，以鱼苗为饵料，至15 DPH为饵料的组（C组）pomc和cck表达水平显著高于其他组（P< 0.05）。A组和D组中amy、ctrb和pl的表达量均显著高于A组和D组，pepp的表达量均显著高于A组和D组。与肠道发育和成熟相关的两个基因（alp、lap 3和anpep）在卤虫和饵料组合组中的表达量最高，但与A组相比差异不显著。在幼体饲料中，卤虫与饵料组合（D组）的吸收和生长（mtor、pept 1和digf-1）的表达增加，表明消化结构的功能更有效。因此，卤虫与饵料鱼苗配合投喂，可以获得与饵料鱼苗相当的生长和较好的成活率，是一种经济的鲈鱼仔鱼初投喂方式。
This study investigates the effects of first feeding regime on growing, survival, and gene expression (appetite, digestion, absorption, and growth) and digestive enzymes activity in Chinese perch (Siniperca chuatsi) larvae. The fish larvae at 3 days post-hatch (DPH), were fed with prey fry (newly hatched zebrafishDanio rerio)/Artemianaupli from 3 to 15 DPH for different time periods as follows: (A)only prey fry;(B) 3–5 DPHArtemia/6–15 DPH prey fry;(C) 3–7 DPHArtemia/8–15 DPH prey fry; (D) a combination ofArtemiaand prey fry. Final performance parameters (total length, body weight, survival rate), gene expression and digestive enzymes were measured and compared among treatments at 15 DPH. The groups fed only prey fry (group A) (53.67 ± 1.76%) and combination ofArtemiaand prey fry (group D) (61.22 ± 1.18%), respectively, showed significantly (P< 0.05) higher survival specific growth rate (SGRTL) than other groups. Brain expression of npy and agrp were not significantly affected under different feeding regimes. The groups fed withArtemiatill 7 DPH followed by feeding with prey fry till 15 DPH (group C) showed significantly (P< 0.05) higher pomc and cck expression level than the other groups. The expression ofamy,ctrb, andplwas significantly higher in larvae fed combination ofArtemiaand prey fry (group D).pephad a high expression level in both group A and D. The highest expression of two genes related to intestinal development and maturation (alp, lap3,anpep) were observed in the fish fed a combination ofArtemiaand prey fry, but there was no significant difference compared to group A. The expression of absorption and growth (mtor,pept1andigf-1) increased with combination ofArtemiaand prey fry in larval diet (group D), indicating a more efficient functionality of digestive structures. In conclusion, feeding with combination ofArtemiaand prey fry could be suggested as an economical feeding regime for first feeding of Chinese perch larvae as comparable growth to prey fry were achieved, and better survival.