PYRUVATE AND GLUCOSE-UPTAKE BY MOUSE OVA AND PREIMPLANTATION EMBRYOS
PYRUVATE AND GLUCOSE-UPTAKE BY MOUSE OVA AND PREIMPLANTATION EMBRYOS
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DOI：
10.1530/jrf.0.0720009
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
BARTON, AM
中科院分区：
文献类型：
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作者：
LEESE, HJ;BARTON, AM
已经设计了一种方法来测量少量小鼠卵子和植入前胚胎对营养物质的吸收。将1-5个胚胎作为一组在37℃下孵育。 C 在矿物油下的硅化显微镜载玻片上的小体积（至少 20 nl/胚胎）培养基中。在 3 小时内以 30 分钟的间隔采集 4 nl 连续样品，并使用超微荧光技术测定其葡萄糖和丙酮酸的含量。在未受精和受精的卵子以及囊胚的发育阶段，丙酮酸的吸收超过了葡萄糖的吸收，此时葡萄糖成为主要底物。未受精卵吸收丙酮酸的一个组成部分可能是由载体介导的。
A method has been devised to measure the uptake of nutrients by small numbers of mouse ova and preimplantation embryos. The 1-5 embryos were incubated as a group at 37.degree. C in a small volume (at least 20 nl/embryo) of culture medium on siliconized microscope slides under mineral oil. Serial 4 nl samples were taken at 30-min intervals for 3 h and assayed for their content of glucose and pyruvate using an ultramicrofluorometric technique. Pyruvate uptake exceeded that of glucose in unfertilized and fertilized ova and in the development stages up to the blastocyst, when glucose became the predominant substrate. A component of the uptake of pyruvate by unfertilized ova was probably mediated by a carrier.