Quinoxyfen perturbs signal transduction in barley powdery mildew (Blumeria graminis f.sp hordei)
Quinoxyfen perturbs signal transduction in barley powdery mildew (Blumeria graminis f.sp hordei)
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DOI：
10.1046/j.1364-3703.2003.00165.x
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发表时间：
2003-05-01
影响因子：
4.9
通讯作者：
Gurr, SJ
中科院分区：
文献类型：
--
作者：
Wheeler, IE;Hollomon, DW;Gurr, SJ
喹诺芬是一种保护性杀菌剂，通过干扰萌发和/或附着胞的形成来控制白粉病。大麦白粉病的突变体。与大量的野生型孢子相比，对喹诺芬有抗性的孢子产生较少的分生孢子，分生孢子的萌发和附着胞的形成更加混杂。这表明抵抗绕过宿主识别信号。在野生型胚种形态发生过程中记录的信号转导基因的RT-PCR图谱显示，喹诺芬改变了蛋白激酶C （pkc）、pkc样和蛋白激酶A （cpka）催化亚基转录物的积累。差异显示-反转录PCR在野生型分生孢子中发现了一个基因转录物，而在喹诺芬抗性突变体的分生孢子中不存在或含量少得多。在大麦上接种野生型分生孢子24 h后，该mRNA未被检测到。它编码GTP酶激活蛋白（GAP），该蛋白可与小分子量ras型GTP结合蛋白相互作用。在喹诺芬存在的情况下，缺口mRNA在整个胚胚形态发生过程中仍然存在。GAP参与耐药性表明喹诺芬通过破坏早期细胞信号事件抑制霉菌感染。
Quinoxyfen is a protectant fungicide which controls powdery mildew diseases by interfering with germination and/or appressorium formation. Mutants of barley powdery mildew, Blumeria graminis f.sp. hordei, which are resistant to quinoxyfen produce fewer conidia, which germinate and form appressoria more promiscuously than do the prolific numbers of wild-type spores. This suggests that resistance bypasses host recognition signals. RT-PCR profiles of signal transduction genes, recorded during wild-type germling morphogenesis, reveals that quinoxyfen alters the accumulation of Protein Kinase C (pkc), pkc-like and catalytic subunit of Protein Kinase A (cpka) transcripts. Differential display-reverse transcription PCR identified a gene transcript in wild-type conidia that was absent, or much less abundant, in conidia from quinoxyfen-resistant mutants. This mRNA was not detectable 24 h after wild-type conidia were inoculated on to barley. It encodes a GTPase activating protein (GAP), which may interact with a small molecular weight Ras-type GTP binding protein. In the presence of quinoxyfen, the gap mRNA remains throughout germling morphogenesis. The involvement of GAP in resistance suggests that quinoxyfen inhibits mildew infection by disrupting early cell signalling events.