Is functional state of spinal microglia involved in the anti-allodynic and anti-hyperalgesic effects of electroacupuncture in rat model of monoarthritis?
Is functional state of spinal microglia involved in the anti-allodynic and anti-hyperalgesic effects of electroacupuncture in rat model of monoarthritis?
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DOI：
10.1016/j.nbd.2007.02.007
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发表时间：
2007-06-01
影响因子：
6.1
通讯作者：
Zhang Yu-Qiu
中科院分区：
文献类型：
--
作者：
Shan, Sun;Qi-Liang, Mao-Ying;Zhang Yu-Qiu
脊髓小胶质细胞在组织炎症或损伤后造成夸大的疼痛中起着关键作用。电针可以有效地控制炎症性疾病患者和实验性炎症性疼痛动物的夸大疼痛。然而，脊髓神经胶质细胞激活与电针镇痛之间的关系却知之甚少。通过免疫组织化学、RT-PCR分析和行为学测试，本研究表明：(1)单侧关节内注射CFA可显著激活小胶质细胞，上调脊髓组织中肿瘤坏死因子(TNF)-α、白介素1β(IL-1β)和白介素6(IL-6)的表达水平；(2)反复鞘内注射(I.T.)注射小胶质细胞抑制剂米诺环素(100 MU G)或电针同侧“环跳穴”、“阳陵泉”穴可显著抑制CFA诱导的伤害性行为超敏反应和脊髓小胶质细胞的激活；(3)电针联合米诺环素可显著增强电针对痛觉过敏和痛觉过敏的抑制作用。这些数据首次为脊髓小胶质细胞功能状态参与电针抗伤害性反应提供了直接证据。因此，电针的抗神经炎作用可能是其抗关节炎疼痛作用的机制之一，从而可能提出一种多学科综合治疗关节炎相关症状的方法。(C)2007 Elsevier Inc.保留所有权利。
Spinal microglia play a key role for creating exaggerated pain following tissues inflammation or injury. Electroacupuncture (EA) can effectively control the exaggerated pain both in humans with inflammatory disease and animals with experimental inflammatory pain. However, little is known about the relationship between spinal glial activation and EA analgesia. Using immunohistochemistry, RT-PCR analysis, and behavioral testing, the present study demonstrated that (1) Unilateral intraarticular injection of CFA produced a robust microglial activation and the up-regulation of the tumor necrosis factor (TNF)-alpha, interleukin (IL-1 beta), and IL-6 mRNA levels in the spinal cord; (2) Repeated intrathecal (i.t.) injection of minocycline (100 mu g), a microglial inhibitor, or EA stimulation of ipsilateral "Huantiao"(GB30) and "Yang-lingquan" (GB34) acupoints significantly suppressed CFA-induced nociceptive behavioral hypersensitivity and spinal microglial activation; (3) Combination of EA with minocycline significantly enhanced the inhibitory effects of EA on allodynia and hyperalgesia. For the first time, these data provide direct evidence for the involvement of spinal microglial functional state in anti- nociception of EA. Thus, anti-neuroinflammatory effect of EA might be considered as one of the mechanisms of its anti-arthritic pain effects, and thereby a multidisciplinary integrated approach to treating symptoms related to arthritis might be raised. (c) 2007 Elsevier Inc. All rights reserved.