Nkd2 promotes the differentiation of dental follicle stem/progenitor cells into osteoblasts.
Nkd2 promotes the differentiation of dental follicle stem/progenitor cells into osteoblasts.
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Nkd2促进牙囊干/祖细胞分化为成骨细胞
DOI：
10.3892/ijmm.2018.3822
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发表时间：
2018-11
影响因子：
5.4
通讯作者：
Hou Y
中科院分区：
文献类型：
--
作者：
Chen C;Zhang J;Ling J;Du Y;Hou Y
牙囊干/祖细胞具有成骨潜能。裸角质层同源物2（Nkd 2）是经典Wnt信号传导途径的信号诱导反馈拮抗剂。本研究旨在探讨Nkd 2在牙囊干/祖细胞（DFSCs）向成骨细胞分化中的作用。采用免疫组化、逆转录-定量聚合酶链反应和蛋白质印迹法检测大鼠DFSCs中Nkd 2的表达。此外，用小干扰RNA转染大鼠DFSC（rDFSC），以检查Nkd 2对这些细胞分化成成骨细胞的影响。此外，使用β-catenin/T细胞因子荧光素酶活性测定和蛋白质印迹法研究了Nkd 2在rDFSC中Wnt/β-catenin途径中的功能。结果显示，在rDFSCs向成骨细胞分化的过程中，Nkd 2的表达上调。与si-NC组相比，Nkd 2沉默的rDFSCs成骨分化能力和Wnt/β-catenin通路活性显著降低（分别为P<0.05和P<0.001）。结果表明Nkd 2通过Wnt/β-catenin信号通路促进rDFSCs向成骨细胞分化。
Dental follicle stem/progenitor cells have the potential to undergo osteogenesis. naked cuticle homolog 2 (Nkd2) is a signal-inducible feedback antagonist of the canonical Wnt signaling pathway. The purpose of the present study was to investigate the function of Nkd2 in the differentiation of dental follicle stem/progenitor cells (DFSCs) into osteoblasts. Immunohistochemistry, reverse transcription-quantitative polymerase chain reaction and western blotting were employed to detect Nkd2 expression in rat DFSCs. In addition, rat DFSCs (rDFSCs) were transfected with small interfering RNAs to examine the effect of Nkd2 on the differentiation of these cells into osteoblasts. Furthermore, the function of Nkd2 in the Wnt/β-catenin pathway in rDFSCs was investigated using β-catenin/T-cell factor luciferase activity assays and western blotting. It was revealed that the expression of Nkd2 was upregulated during the differentiation of rDFSCs into osteoblasts. Furthermore, osteoblast differentiation ability and Wnt/β-catenin pathway activity were significantly decreased in Nkd2-silenced rDFSCs compared with the si-NC group (P<0.05 and P<0.001, respectively). The results suggest that Nkd2 promotes the differentiation of rDFSCs into osteoblasts through Wnt/β-catenin signaling.
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影响因子：
7.6
作者：
Liu F;Millar SE
通讯作者：
Millar SE
影响因子：
37.3
作者：
Hu T;Li C
通讯作者：
Li C
DOI：
10.1016/j.bbrc.2011.07.116
发表时间：
2011-09-09
影响因子：
3.1
作者：
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通讯作者：
Cho, Eui-Sic
影响因子：
4.1
作者：
Nemoto, Eiji;Koshikawa, Yohei;Shimauchi, Hidetoshi
通讯作者：
Shimauchi, Hidetoshi
影响因子：
4.8
作者：
Gaur, T;Lengner, CJ;Lian, JB
通讯作者：
Lian, JB