Changes in cytokine production and T cell subpopulations in experimentally induced zinc-deficient humans
Changes in cytokine production and T cell subpopulations in experimentally induced zinc-deficient humans
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DOI：
10.1152/ajpendo.1997.272.6.e1002
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发表时间：
1997-06-01
影响因子：
5.1
通讯作者：
Brewer, GJ
中科院分区：
文献类型：
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作者：
Beck, FWJ;Prasad, AS;Brewer, GJ
我们利用人类缺锌的实验模型研究TH 1和TH 2细胞的细胞因子产生。此外，我们测定了CD 4+与CD 8+和CD 4 + CD 45 RA+与CD 4 + CD 45 RO+细胞的比率以及CD 8+亚群中CD 73 + T细胞溶解性细胞的百分比。在基线期间、限锌期结束时和锌补充后收集数据。我们的研究结果表明，TH 1细胞的功能，如干扰素-γ，白细胞介素-2（IL-2），和肿瘤坏死因子-α的生产证明，下降，而TH 2细胞的功能（生产IL-4，IL-6和IL-10）不受锌缺乏的影响。因此，由于人类缺锌，导致TH 1和TH 2细胞之间的失衡。我们的研究还表明，锌可能需要新的CD 4 + T淋巴细胞的再生和T细胞溶解细胞的维持。我们的结论是，TH 1和TH 2细胞之间的不平衡，减少招募的T幼稚细胞，减少百分比的T细胞溶解性细胞可能占细胞介导的免疫功能降低锌缺乏的主题。
We have utilized an experimental model of human zinc deficiency for study of cytokines production by TH1 and TH2 cells. Additionally, we determined ratios of CD4+ to CD8+ and CD4+ CD45RA+ to CD4+CD45RO+ cells and percentages of CD73+ T cytolytic cells in the CD8+ subset. The data were collected during baseline, at the end of the zinc-restricted period, and following zinc repletion. Our results showed that functions of TH1 cells, as evidenced by production of interferon-gamma, interleukin-2 (IL-2), and tumor necrosis factor-alpha, were decreased, whereas functions of TH2 cells (production of IL-4, IL-6, and IL-10) were unaffected by zinc deficiency. Thus an imbalance between TH1 and TH2 cells resulted because of zinc deficiency in humans. Our studies also showed that zinc may be required for regeneration of new CD4+ T lymphocytes and maintenance of T cytolytic cells. We conclude that an imbalance between TH1 and TH2 cells, decreased recruitment of T naive cells, and decreased percentage of T cytolytic cells may account for decreased cell-mediated immune functions in zinc-deficient subjects.