Flavobacterium palustre sp. nov., isolated from wetland soil.
Flavobacterium palustre sp. nov., isolated from wetland soil.
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DOI：
10.1099/ijs.0.000054
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发表时间：
2015-03
影响因子：
2.8
通讯作者：
Hao Feng;Yanhua Zeng;Yili Huang
中科院分区：
文献类型：
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作者：
Hao Feng;Yanhua Zeng;Yili Huang
从浙江西溪湿地岸边土壤中分离到一株革兰氏染色阴性、不运动、黄色杆状细菌，命名为S44（T）。在6-37 °C（最适，28 °C）和pH 6.0-9.0（最适，7.0）下观察到菌株S44（T）的生长。在> 2%（w/v）NaCl的存在下没有发生生长。基于16 S rRNA基因序列的系统发育分析显示，菌株S44（T）代表黄杆菌属的成员，显示出与来自黄杆菌琥珀酸DSM 4002（T）（96.9%）、赖氏黄杆菌WB 3.2-61（T）（96.6%）和大豆黄杆菌NCBI 105008（T）（96.5%）的序列的最高序列相似性。基因组DNA的G+C含量为33.6mol%。主要的细胞脂肪酸是C15：0、iso-C15：0、antiso-C15：0和总和特征3（包括C16：1ω 7 c和/或iso-C15：0 2-OH），并且主要的呼吸醌是甲基萘醌6（MK-6）。主要极性脂质为磷脂酰乙醇胺、2种未知氨基脂质、2种未知氨基磷脂和4种未知极性脂质。根据表型和基因型数据，建议将分离物S44（T）分类为代表黄杆菌属的一个新种，并建议将其命名为沼泽黄杆菌新种。模式菌株为S44（T）（= CGMCC 1.12811（T）= NBRC 110389（T））。
A Gram-staining-negative, non-motile, yellow-coloured, rod-shaped bacterium, designated S44(T), was isolated from bankside soil of Xixi wetland, located in Zhejiang province, China. Growth of strain S44(T) was observed at 6-37 °C (optimum, 28 °C) and at pH 6.0-9.0 (optimum, 7.0). No growth occurred in the presence of >2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S44(T) represented a member of the genus Flavobacterium, showing the highest sequence similarities to the sequences from Flavobacterium succinicans DSM 4002(T) (96.9 %), Flavobacterium reichenbachii WB 3.2-61(T) (96.6 %) and Flavobacterium glycines NCBI 105008(T) (96.5 %). The G+C content of the genomic DNA was 33.6 mol%. The predominant cellular fatty acids were C15 : 0, iso-C15 : 0, anteiso-C15 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH), and the major respiratory quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unknown aminolipids, two unknown aminophospholipids and four unknown polar lipids. On the basis of the phenotypic and genotypic data, it is proposed that the isolate S44(T) be classified as representing a novel species of the genus Flavobacterium, for which the name Flavobacterium palustre sp. nov. is proposed. The type strain is S44(T) ( = CGMCC 1.12811(T) = NBRC 110389(T)).