Sustained protein therapeutics enabled by self-healing nanocomposite hydrogels for non-invasive bone regeneration
Sustained protein therapeutics enabled by self-healing nanocomposite hydrogels for non-invasive bone regeneration
复制标题
通过自愈纳米复合水凝胶实现持续的蛋白质治疗，用于非侵入性骨再生
DOI：
10.1039/c9bm01455a
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发表时间：
2020
影响因子：
6.6
通讯作者：
Weian Zhang
中科院分区：
文献类型：
--
作者：
Yuanhao Zhang;Mingjiao Chen;Zhaobo Dai;Hongliang Cao;Jin Li;Weian Zhang
Bone tissue engineering based on stem cells, growth factors and bioactive scaffolds presents an appealing but challenging approach for rehabilitation of patients with bone defects. A versatile system with the capability for easy operation and precise protein delivery in specific locations is attractive for enhancing bone regeneration. Here, we develop a non-invasive delivery system based on injectable and self-healing nanocomposite hydrogels for sustained protein release, which has the potential to improve the current orthopedic strategy. Specifically, LAPONITE® (LAP) nanoplatelets are able to accelerate the gelation process through hydrogen bonds with polysaccharide matrices, endowing hydrogels with superior mechanical and rheological behaviors, along with better injectability and self-healing ability. Attractively, the strong static binding between LAP nanoplatelets and bone morphogenetic protein-2 (BMP-2) can form stable LAP@BMP-2 complexes. The results indicate that the complexes effectively preserve the intrinsic bioactivity of BMP-2 and prolong the release period for more than four weeks. Moreover, hydrogels incorporating with the LAP@BMP-2 complexes synergistically boost cell spreading, proliferation activity and osteogenesis, both in vitro and in vivo, compared with LAP or BMP-2 alone. Overall, this study proposes a valid platform for protein therapeutics and non-invasive bone repair.
