Localization and developmental expression of mRNA for cortical rod protein in kuruma prawn Marsupenaeus japonicus
Localization and developmental expression of mRNA for cortical rod protein in kuruma prawn Marsupenaeus japonicus
复制标题
DOI：
10.2108/zsj.22.675
复制
发表时间：
2005-06-01
影响因子：
0.9
通讯作者：
Aida, K
中科院分区：
文献类型：
--
作者：
Kim, YK;Tsutsui, N;Aida, K
成熟的对虾卵母细胞具有包含两个相关的皮质棒蛋白（CRIP，28.6 kDa和30.5 kDa）的皮质棒。本研究以虾类养殖中的重要经济品种--日本对虾（Marsupenaeus）为研究对象，研究了卵巢发育过程中CRP mRNA的定位以及CRP和卵黄蛋白原（Vg）的基因表达谱。北方印迹分析显示卵巢中有CRP mRNA表达。原位杂交显示，在未成熟和成熟卵巢的早期发育阶段的卵母细胞中的CRP转录物的强信号。实时定量分析聚合酶链反应显示，CRP mRNA水平较高的previtellogenic和内源性（初级）卵黄发生阶段比更先进的阶段。与CRP mRNA，Vg mRNA水平低，在卵巢和肝胰腺pretellogenic女性。当卵巢发育到内源性卵黄发生期时，卵巢Vg mRNA水平显著升高，后期迅速下降。另一方面，肝胰腺中的Vg mRNA水平在外源性（次级）卵黄发生和成熟阶段往往较高，其中卵巢Vg mRNA水平下降。我们的研究结果表明，CRP mRNA的高表达卵黄发生之前，这表明CRP的转录，翻译和皮质杆的形成发生在卵母细胞发育的不同阶段。内源性卵黄发生阶段是启动CRP和Vg合成的关键阶段。这些蛋白质合成的一致性表明，CRP和Vg的合成受到密切相关的机制的调节。
The mature penaeid oocytes possess cortical rods that contain two related cortical rod proteins (CRIP, 28.6 kDa and 30.5 kDa). In the present study, localization of CRP mRNA and gene expression profiles of CRP and vitellogenin (Vg) during ovarian development were examined in kuruma prawn, Mar-supenaeus japonicus, an economically important species for shrimp and prawn farming. Northern blot analysis revealed that CRP mRNA was expressed in the ovary. In situ hybridization showed strong signals for CRP transcripts in the oocytes at early developmental stages in both immature and mature ovaries. Quantitative analysis by real-time polymerase chain reaction revealed that CRP mRNA levels were higher in the previtellogenic and endogenous (primary) vitellogenic stages than in more advanced stages. Unlike CRP mRNA, Vg mRNA levels were low in the ovary and hepatopancreas in previtellogenic females. When the ovary developed into the endogenous vitellogenic stage, ovarian Vg mRNA levels increased significantly, followed by rapid decrease in more advanced stages. The Vg mRNA levels in the hepatopancreas, on the other hand, tended to be high in the exogenous (secondary) vitellogenic and maturation stages, in which ovarian Vg mRNA levels were decreased. Our findings indicate that CRP mRNA is highly expressed before the onset of vitellogenesis, suggesting that the transcription, translation, and cortical-rod formation of CRP occur at different phases of oocyte development. The endogenous vitellogenic stage is a crucial stage for the initiation of CRP and Vg syntheses. The coincidence of these protein syntheses suggests that CRP and Vg syntheses are regulated by closely-related mechanisms.