Parallel Accelerator and Molecular Mass Spectrometry Measurement of Carbon-14-Labeled Analytes.
Parallel Accelerator and Molecular Mass Spectrometry Measurement of Carbon-14-Labeled Analytes.
复制标题
碳 14 标记分析物的并行加速器和分子质谱测量。
DOI：
10.1007/978-1-0716-1585-0_1
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Ognibene,TedJ
中科院分区：
文献类型：
--
作者：
Stewart,BenjaminJ;Ognibene,TedJ
并行加速器分子质谱（PAMMS）是一种能够同时定量14 C示踪物和表征14 C标记生物分子结构的强大分析技术。在这里，我们描述了使用PAMS的分析生物分子分离的高效液相色谱法。本方案旨在为需要使用资源中心（如劳伦斯利弗莫尔国家实验室的生物加速器质谱国家用户资源）提供的仪器进行PAMMS实验的研究人员提供指导。
Parallel accelerator and molecular mass spectrometry (PAMMS) is a powerful analytical technique capable of simultaneous quantitation of carbon-14 tracer and structural characterization of14C-labeled biomolecules. Here we describe the use of PAMMS for the analysis of biological molecules separated by high-performance liquid chromatography. This protocol is intended to serve as a guide for researchers who need to perform PAMMS experiments using instrumentation available at resource centers such as the National User Resource for Biological Accelerator Mass Spectrometry at Lawrence Livermore National Laboratory.