Fermented Maesil (Prunus mume) with probiotics inhibits development of atopic dermatitis-like skin lesions in NC/Nga mice
Fermented Maesil (Prunus mume) with probiotics inhibits development of atopic dermatitis-like skin lesions in NC/Nga mice
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DOI：
10.1111/j.1365-3164.2009.00796.x
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发表时间：
2010-04-01
影响因子：
1.4
通讯作者：
Lee, Bong-Joo
中科院分区：
文献类型：
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作者：
Jung, Bock-Gie;Cho, Sun-Ju;Lee, Bong-Joo
梅（Prunus mume Siebold & Zucc.），作为自由基清除剂和促炎介质抑制剂的潜在来源，在传统的韩国药物制剂中用作皮肤病的治疗，如益生菌。在NC/Nga小鼠模型中确定益生菌发酵的Maesil制剂对特应性皮炎（AD）样皮肤病变发展的作用，作为开发用于狗的治疗性饲料补充剂的初始步骤。连续摄入实验饲料显著抑制AD样皮肤病变的发展，如皮肤体征显著减少和皮肤病变内炎症减少所证明的。通过嗜酸性粒细胞比率和血清IgE浓度的显著降低以及从耳中回收的金黄色葡萄球菌数量的减少证实了疗效。与对照组相比，实验动物脾脏中IL-4、干扰素-γ和肿瘤坏死因子-α的相对mRNA表达水平也降低，IL-10的血清浓度升高，同时IL-4浓度降低。总之，结果表明发酵Maesil的一些组分具有抑制AD样皮肤病变发展的能力，可能是通过刺激IL-10。因此，预期发酵Maesil对AD犬的有益作用，尽管这一点和活性途径的性质仍有待探索。
Maesil (Prunus mume Siebold & Zucc.), a potential source of free radical scavengers and inhibitor of pro-inflammatory mediators, is used in traditional Korean medical preparations as a remedy for skin disorders as have probiotics. The action of a probiotic fermented Maesil preparation on the development of atopic dermatitis (AD)-like skin lesions was determined in a NC/Nga mouse model as an initial step towards the development of a therapeutic feed supplement for use in dogs. Continuous ingestion of the experimental feed markedly inhibited the development of the AD-like skin lesions, as evidenced by a marked decrease in skin signs and reduced inflammation within the skin lesions. Efficacy was confirmed by significant decreases in eosinophil ratio and serum IgE concentration, and a reduction in the number of Staphylococcus aureus recovered from the ear. Relative mRNA expression levels of IL-4, interferon-gamma and tumour necrosis factor-alpha in the spleens of the experimental animals were also decreased and there was an increased serum concentration of IL-10 with a concurrent decreased IL-4 concentration in comparison to a control group. Taken together, the results indicate that some component(s) of fermented Maesil have the ability to suppress the development of AD-like skin lesions, possibly by stimulation of IL-10. Beneficial effects of fermented Maesil may thus be expected in dogs with AD, although this and the nature of the active pathway remain to be explored.