Lung stem and progenitor cells in tissue homeostasis and disease.
Lung stem and progenitor cells in tissue homeostasis and disease.
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DOI：
10.1016/b978-0-12-416022-4.00008-1
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发表时间：
2014
影响因子：
--
通讯作者：
Kim, Carla F.
中科院分区：
文献类型：
--
作者：
Leeman, Kristen T.;Fillmore, Christine M.;Kim, Carla F.
哺乳动物的肺是一个复杂的器官，含有许多推定的干/祖细胞群体，有助于区域特异性组织稳态和修复。在这篇综述中，我们讨论了在肺内稳态和疾病的背景下识别和研究这些细胞群的最新进展。基因工程小鼠现在允许在不同类型的肺损伤修复期间在体内追踪几种肺干细胞和祖细胞群体的谱系。使用特定的细胞表面标志物组，这些细胞也可以从鼠和人肺中分离，并在3D培养系统和体内移植测定中进行测试。包括肺癌在内的毁灭性肺部疾病的病理学可能部分归因于肺干细胞的失调和功能障碍。通过鉴定新的、独特的标记物，更精确地表征干细胞;改进分离和移植技术;以及进一步开发功能测定，最终将为许多人类肺部疾病带来新的治疗方法。特别是，肺癌生物学可能极大地受到正常肺干细胞生物学发现的影响，因为有证据表明肺癌是一种始于肿瘤性肺干细胞并可能由肿瘤性肺干细胞驱动的疾病。
The mammalian lung is a complex organ containing numerous putative stem/progenitor cell populations that contribute to region-specific tissue homeostasis and repair. In this review, we discuss recent advances in identifying and studying these cell populations in the context of lung homeostasis and disease. Genetically engineered mice now allow for lineage tracing of several lung stem and progenitor cell populations in vivo during different types of lung injury repair. Using specific sets of cell surface markers, these cells can also be isolated from murine and human lung and tested in 3D culture systems and in vivo transplant assays. The pathology of devastating lung diseases, including lung cancers, is likely in part due to dysregulation and dysfunction of lung stem cells. More precise characterization of stem cells with identification of new, unique markers; improvement in isolation and transplant techniques; and further development of functional assays will ultimately lead to new therapies for a host of human lung diseases. In particular, lung cancer biology may be greatly informed by findings in normal lung stem cell biology as evidence suggests that lung cancer is a disease that begins in, and may be driven by, neoplastic lung stem cells.