Proteomic study of salivary peptides and proteins in patients with Sjogren's syndrome before and after pilocarpine treatment
Proteomic study of salivary peptides and proteins in patients with Sjogren's syndrome before and after pilocarpine treatment
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DOI：
10.1002/art.22738
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发表时间：
2007-07-01
影响因子：
--
通讯作者：
Ferraccioli, G. F.
中科院分区：
文献类型：
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作者：
Peluso, G.;De Santis, M.;Ferraccioli, G. F.
Objective.研究毛果芸香碱对原发性干燥综合征（SS）患者唾液肽和蛋白质谱的影响，并研究原发性SS患者、SS合并其他风湿性疾病患者和健康对照者之间的差异。唾液标本来自9名原发性SS患者，9名继发性SS患者和10名健康对照。使用配备电喷雾电离源的光谱仪，使用高效液相色谱法结合质谱法分析样品中62种不同唾液蛋白的水平。在6例原发性SS患者中，在服用5 mg毛果芸香碱后30 min、60 min和24 h收集唾液。在毛果芸香碱之前，原发性SS患者样本中类似于60%的唾液蛋白无法识别或水平低于健康对照组。在毛果芸香碱治疗后30-60分钟后，在原发性SS患者和对照组中发现了约三分之一的较少代表的蛋白质。与对照组相比，在原发性SS患者中以较低水平检测到的几乎所有蛋白质在毛果芸香碱后30-60分钟达到与对照组相似的水平。腮腺蛋白对毛果芸香碱的反应最好。原发性SS患者的特征在于较高的α-防御素I水平和β-防御素2的存在。继发性SS患者的蛋白质谱介于原发性SS患者和对照组之间。毛果芸香碱部分恢复了原发性SS患者唾液中可识别蛋白的水平和数量。原发性SS患者唾液中较高水平的β-防御素1和β-防御素2可能是该患者组口腔炎症的标志物。
Objective. To investigate the effect of pilocarpine on the salivary peptide and protein profile in patients with primary Sjogren's syndrome (SS) and to study the differences between patients with primary SS, patients with SS associated with other rheumatic diseases, and healthy control subjects.Methods. Saliva specimens were obtained from 9 primary SS patients, 9 secondary SS patients, and 10 healthy controls. Samples were analyzed for levels of 62 different salivary proteins using high-performance liquid chromatography coupled with mass spectrometry using a spectrometer equipped with an electrospray ionization source. In 6 of the primary SS patients, saliva was collected at 30 minutes, 60 minutes, and 24 hours after taking 5 mg of pilocarpine.Results. Before pilocarpine, similar to 60% of salivary proteins in samples from primary SS patients were not identifiable or showed lower levels than those in healthy controls. After 30-60 minutes following pilocarpine treatment, approximately one-third of the less represented proteins was found in a similar percentage of primary SS patients and controls. Almost all of the proteins that were detectable at lower levels in primary SS patients compared with controls reached levels similar to those in controls at 30-60 minutes after pilocarpine. The parotid gland proteins had the best response to pilocarpine. Primary SS patients were characterized by higher a-defensin I levels and by the presence of beta-defensin 2. Secondary SS patients showed an intermediate protein profile between that of the primary SS patients and the controls.Conclusion. Pilocarpine partially restored the levels and numbers of identifiable proteins in saliva from patients with primary SS. Higher levels of beta-defensin 1 and the presence of beta-defensin 2 in the saliva of patients with primary SS could be markers of oral inflammation in this patient group.