Fukuda T, Haraguchi A, Takahashi M, Nakaoka T, Fukazawa M, Okubo J, Ozaki M, Kanatome A, Ohya R, Miura Y, Obara K, Shibata S.
Fukuda T, Haraguchi A, Takahashi M, Nakaoka T, Fukazawa M, Okubo J, Ozaki M, Kanatome A, Ohya R, Miura Y, Obara K, Shibata S.
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福田 T、原口 A、高桥 M、中冈 T、深泽 M、大久保 J、尾崎 M、加纳美 A、Ohya R、三浦 Y、小原 K、柴田 S。
DOI：
10.1080/07420528.2018.1490315
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发表时间：
2018
影响因子：
2.8
通讯作者：
Shibata S.
中科院分区：
文献类型：
--
作者：
Fukuda T;Haraguchi A;Takahashi M;Nakaoka T;Fukazawa M;Okubo J;Ozaki M;Kanatome A;Ohya R;Miura Y;Obara K;Shibata S.
在哺乳动物中，日常生理活动受昼夜节律的调节，昼夜节律包括两种类型的内部时钟：中央时钟和外围时钟。昼夜节律在维持包括睡眠-觉醒周期、体温、代谢和器官功能在内的生理功能方面起着重要作用。例如，不规律的生活方式或长途旅行引起的昼夜节律紊乱会增加患疾病的风险;因此，正确维持生物钟的节奏很重要。众所周知，食物和生物钟系统密切相关。对啮齿类动物的研究表明，摄入特定的食物成分，如类黄酮、鱼油、多酚白藜芦醇和氨基酸L-鸟氨酸会影响生物钟。然而，关于影响人类生物钟的食物的报道很少。因此，在这项研究中，我们在一项交叉设计的安慰剂对照人体试验中研究了L-鸟氨酸是否影响人类中央时钟。总共有28名健康成人（即≥20岁）被随机分为两组并完成研究方案。在第一个摄入期，要求参与者服用L-鸟氨酸（400 mg）胶囊或安慰剂胶囊7天。间隔7天后，他们在第二次服用期间服用替代试验胶囊7天。在每个摄入期的最后一天，在昏暗的光线条件下在不同的时间点对唾液进行采样，并定量褪黑激素的浓度以评估中央时钟的相位。结果表明，昏暗的光褪黑激素的发病，一个公认的标志，中央昼夜节律相，被推迟15分钟后摄入L-鸟氨酸。这一发现不仅表明L-鸟氨酸影响人类中央生物钟，而且还表明人类中央生物钟可以通过食物成分进行调节。
In mammals, daily physiological events are regulated by the circadian rhythm, which comprises two types of internal clocks: the central clock and peripheral clocks. Circadian rhythm plays an important role in maintaining physiological functions including the sleep-wake cycle, body temperature, metabolism and organ functions. Circadian rhythm disorder, which is caused, for example, by an irregular lifestyle or long-haul travel, increases the risk of developing disease; therefore, it is important to properly maintain the rhythm of the circadian clock. Food and the circadian clock system are known to be closely linked. Studies on rodents suggest that ingesting specific food ingredients, such as the flavonoid nobiletin, fish oil, the polyphenol resveratrol and the amino acid L-ornithine affects the circadian clock. However, there are few reports on the foods that affect these circadian clocks in humans. In this study, therefore, we examined whether L-ornithine affects the human central clock in a crossover design placebo-controlled human trial. In total, 28 healthy adults (i.e. ≥20 years) were randomly divided into two groups and completed the study protocol. In the 1st intake period, participants were asked to take either L-ornithine (400 mg) capsules or placebo capsules for 7 days. After 7 days’ interval, they then took the alternative test capsules for 7 days in the 2nd intake period. On the final day of each intake period, saliva was sampled at various time points in the dim light condition, and the concentration of melatonin was quantified to evaluate the phase of the central clock. The results revealed that dim light melatonin onset, a recognized marker of central circadian phase, was delayed by 15 min after ingestion of L-ornithine. Not only is this finding an indication that L-ornithine affects the human central clock, but it also demonstrates that the human central clock can be regulated by food ingredients.