Dysregulated lipidome of sebum in patients with atopic dermatitis
Dysregulated lipidome of sebum in patients with atopic dermatitis
复制标题
作者：
Yin，Huibin;Qiu，Zhuoqiong;Li，Wei
研究背景脂质是皮肤屏障的主要成分，主要由角质形成细胞和皮脂腺产生。以往的研究屏障功能障碍的特应性皮炎（AD）主要集中在角质形成细胞的脂质，而脂肪腺衍生的脂质在AD的作用一直underrecognized.MethodsThe皮脂分泌的AD患者的皮肤表面上使用Delfin皮脂量表测量。使用Sebutape贴片收集皮脂，并进行液相色谱串联质谱（LC-MS/MS）分析。多变量数据分析应用于探讨脂质体，临床特征，和皮脂腺相关分子之间的关系。ResultsThe量的皮脂腺分泌减少AD患者，并与屏障功能和疾病严重程度呈负相关。LC-MS/MS显示，AD患者和健康人的皮脂脂质体明显聚集。在不同的脂质亚类中，甘油三酯（TG）仅在AD患者中降低，并与疾病严重程度相关。AD患者的第一主成分评分（代表脂质组的主要特征）与SCORAD评分呈正相关，并且在具有不同临床症状（如皮肤干燥和瘙痒）的患者组之间存在显著差异。对先前发表的转录组数据的进一步分析揭示了AD患者非皮损皮肤中脂质代谢相关基因的异常表达，其与皮肤炎症和屏障功能障碍相关，主要来源于内根鞘角质形成细胞和皮脂腺细胞。提示来自皮脂腺的脂质在AD的发病机制中可能起作用。
BackgroundLipids are the major components of skin barrier, mainly produced by keratinocytes and sebaceous glands. Previous studies on barrier dysfunction of atopic dermatitis (AD) mainly focus on the lipids from keratinocytes, whereas the role of sebaceous gland‐derived lipids in AD has long been underrecognized.MethodsThe sebum secreted on the skin surface of AD patients was measured using the Delfin Sebum Scale. Sebum was collected using Sebutape patches and subjected for liquid chromatography tandem‐mass spectrometry (LC–MS/MS) analysis. Multivariate data analysis was applied to explore the relationship among the lipidome, clinical features, and sebaceous gland‐related molecules.ResultsThe amount of sebum secreted from sebaceous glands was decreased in AD patients and was negatively correlated with the barrier function and disease severity. LC–MS/MS revealed the lipidome of sebum, which clustered distinctly between AD patients and healthy individuals. Among the differential lipid subclasses, triglycerides (TG) were exclusively decreased in AD patients and correlated with disease severity. The first principal component scores of AD patients, which represented the main signature of the lipidome, were positively correlated with the SCORAD scores and were significantly different across the patient groups with differential clinical symptoms such as skin dryness and pruritus. Further analysis on the previously published transcriptome data revealed aberrant expression of lipid metabolism‐related genes in non‐lesional skin of AD patients, which was associated with skin inflammation and barrier dysfunction and mainly derived from inner root sheath keratinocytes and sebaceous gland cells.ConclusionAtopic dermatitis patients demonstrated a deviated lipidome of sebum and aberrant lipid metabolism in sebaceous glands, indicating a possible role of lipids from sebaceous glands in the pathogenesis of AD.