Protein Microarrays with Novel Microfluidic Methods: Current Advances.
Protein Microarrays with Novel Microfluidic Methods: Current Advances.
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DOI：
10.3390/microarrays3030180
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发表时间：
2014-07-01
期刊：
影响因子：
--
通讯作者：
Aguirre GR
中科院分区：
文献类型：
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作者：
Dixit CK;Aguirre GR
基于微流体的微镶嵌技术允许以高精度在受限的微米尺度区域中对识别元件进行图案化。这种受控的图案化使得能够开发高度多路复用的阵列多分析物检测。这种阵列技术于2001年初首次引入，具有革命性的微阵列开发和分析物检测的巨大潜力。后来，开发了几种用于微阵列应用的微流体方法。在这篇综述中，我们讨论了这些新的方法和途径，利用微流控技术的属性，显着改善微阵列技术的各个物理方面，如增强印迹均匀性，固定化生物分子的稳定性，减少测定时间，降低成本和笨重的仪器。
Microfluidic-based micromosaic technology has allowed the pattering of recognition elements in restricted micrometer scale areas with high precision. This controlled patterning enabled the development of highly multiplexed arrays multiple analyte detection. This arraying technology was first introduced in the beginning of 2001 and holds tremendous potential to revolutionize microarray development and analyte detection. Later, several microfluidic methods were developed for microarray application. In this review we discuss these novel methods and approaches which leverage the property of microfluidic technologies to significantly improve various physical aspects of microarray technology, such as enhanced imprinting homogeneity, stability of the immobilized biomolecules, decreasing assay times, and reduction of the costs and of the bulky instrumentation.