Molecular Signature of Pseudomonas aeruginosa with Simultaneous Nanomolar Detection of Quorum Sensing Signaling Molecules at a Boron-Doped Diamond Electrode.
Molecular Signature of Pseudomonas aeruginosa with Simultaneous Nanomolar Detection of Quorum Sensing Signaling Molecules at a Boron-Doped Diamond Electrode.
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DOI：
10.1038/srep30001
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发表时间：
2016-07-18
影响因子：
4.6
通讯作者：
Glennon JD
中科院分区：
文献类型：
--
作者：
Buzid A;Shang F;Reen FJ;Muimhneacháin EÓ;Clarke SL;Zhou L;Luong JH;O'Gara F;McGlacken GP;Glennon JD
采用硼掺杂金刚石（BDD）电极对2-庚基-3-羟基-4-喹啉酮（PQS）、2-庚基-4-羟基喹啉（HHQ）和绿脓菌素（PYO）进行电分析。PQS及其前体HHQ是铜绿假单胞菌产生的两种重要信号分子，而PYO是一种氧化还原活性毒素，参与致病和致病过程。这种革兰氏阴性和机会性人类病原体与医院获得性感染相关，特别是在免疫力受损的患者中，并且是囊性纤维化（CF）患者发病和死亡的主要原因。早期检测在这种病原体的临床管理中至关重要，已建立的感染进入生物膜生活方式，这对传统抗生素治疗是难治的。在本文中，优化了检测程序，并证明其用于同时检测标准混合物、生物样品和铜绿假单胞菌掺入的CF痰液样品中的PYO、HHQ和PQS，具有显著的灵敏度，低至纳摩尔水平。差示脉冲伏安法（DPV）扫描也适用于监测铜绿假单胞菌PA 14在培养8小时内的PYO、HHQ和PQS的产生。同时检测到这三种化合物代表了这种病原体特有的分子特征。
Electroanalysis was performed using a boron-doped diamond (BDD) electrode for the simultaneous detection of 2-heptyl-3-hydroxy-4-quinolone (PQS), 2-heptyl-4-hydroxyquinoline (HHQ) and pyocyanin (PYO). PQS and its precursor HHQ are two important signal molecules produced by Pseudomonas aeruginosa, while PYO is a redox active toxin involved in virulence and pathogenesis. This Gram-negative and opportunistic human pathogen is associated with a hospital-acquired infection particularly in patients with compromised immunity and is the primary cause of morbidity and mortality in cystic fibrosis (CF) patients. Early detection is crucial in the clinical management of this pathogen, with established infections entering a biofilm lifestyle that is refractory to conventional antibiotic therapies. Herein, a detection procedure was optimized and proven for the simultaneous detection of PYO, HHQ and PQS in standard mixtures, biological samples, and P. aeruginosa spiked CF sputum samples with remarkable sensitivity, down to nanomolar levels. Differential pulse voltammetry (DPV) scans were also applicable for monitoring the production of PYO, HHQ and PQS in P. aeruginosa PA14 over 8 h of cultivation. The simultaneous detection of these three compounds represents a molecular signature specific to this pathogen.