Unbalanced vitreous levels of pigment epithelium-derived factor and vascular endothelial growth factor in diabetic retinopathy
Unbalanced vitreous levels of pigment epithelium-derived factor and vascular endothelial growth factor in diabetic retinopathy
复制标题
DOI：
10.1016/s0002-9394(02)01568-4
复制
发表时间：
2002-09-01
影响因子：
4.2
通讯作者：
Matsumura, M
中科院分区：
文献类型：
--
作者：
Ogata, N;Nishikawa, M;Matsumura, M
目得：目的：检测糖尿病视网膜病变（DR）患者玻璃体中色素上皮衍生因子（PEDF）和血管内皮生长因子（VEGF）的水平。PEDF和VEGF的水平通过酶联免疫吸附法测定。本文对43例46眼糖尿病视网膜病变行玻璃体切除术患者的玻璃体进行了免疫吸附试验（29例32眼）和特发性黄斑裂孔（MH）（14例14眼）。PEDF的玻璃体浓度显著较低，为1.11 +/- 0.14 μ g/ml（平均值+/-标准误）为1.71 +/- 0.22 μ g/ml（P = 0.021）。DR患者的VEGF水平为1799 +/- 478 μ g/ml，MH患者未检测到。增殖性DR（PDR）的PEDF水平（0.94 +/- 0.12 mug/ml）低于非增殖性DR（NPDR）（2.25 +/- 0.32 mug/ml），活动性DR（0.85 +/- 0.14 mug/ml）显著低于非活动性DR（1.59 +/- 0.24 mug/ml; P = 0.01）。PDR组VEGF水平为2025 ± 533 μ g/ml，NPDR组和活动性DR组VEGF水平为215 ± 201 μ g/ml（2543673 μ g/ml）显著高于非活动期DR（395 +/- 188 mug/ml; P = .0098）。结论：这些结果表明，较低水平的PEDF和较高水平的VEGF可能与DR中的血管生成有关，导致活动性PDR。(C)2002年，Elsevier Science Inc. All rights reserved.
PURPOSE: To determine the levels of pigment epithelium-derived factor (PEDF) and vascular endothelial growth factor (VEGF) in the vitreous of patients with diabetic retinopathy (DR).DESIGN: Experimental study of PEDF and VEGF levels in vitreous samples collected during vitrectomy.METHODS: The levels of PEDF and VEGF were measured by enzyme-linked immunosorbent assay in the vitreous of 46 eyes of 43 patients who underwent vitrectomy with diabetic retinopathy (DR) (32 eyes of 29 patients) and an idiopathic macular hole (MH) (14 eyes of 14 patients).RESULTS: The vitreal concentration of PEDF was significantly lower at 1.11 +/- 0.14 mug/ml (mean +/- standard error) in eyes with DR than in eyes with MH at 1.71 +/- 0.22 mug/ml (P = .021). The VEGF level was 1799 +/- 478 mug/ml in eyes with DR and not detectable in MH. The PEDF level in proliferative DR (PDR) (0.94 +/- 0.12 mug/ml) was lower than that in nonproliferative DR (NPDR) (2.25 +/- 0.32 mug/ml), and that in active DR (0.85 +/- 0.14 mug/ml) was significantly lower than that in inactive DR (1.59 +/- 0.24 mug/ml; P = .01). The VEGF level was 2025 +/- 533 mug/ml in PDR and 215 +/- 201 mug/ml in NPDR and that in active DR (2543 673 mug/ml) was significantly higher than that in inactive DR (395 +/- 188 mug/ml; P = .0098).CONCLUSIONS: These results suggest that lower levels of PEDF and higher levels of VEGF may be related to the angiogenesis in DR that leads to active PDR. (C) 2002 by Elsevier Science Inc. All rights reserved.