Altered gut microbiota in Parkinson's disease patients with motor complications
Altered gut microbiota in Parkinson's disease patients with motor complications
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帕金森病运动并发症患者肠道微生物群的改变
DOI：
10.1016/j.parkreldis.2021.12.012
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Maeda Tetsuya
中科院分区：
文献类型：
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作者：
Takahashi Kai;Nishiwaki Hiroshi;Ito Mikako;Iwaoka Kazuhiro;Takahashi Kenta;Suzuki Yoshio;Taguchi Keita;Yamahara Kanako;Tsuboi Yoshio;Kashihara Kenichi;Hirayama Masaaki;Ohno Kinji;Maeda Tetsuya
前言帕金森病(Parkinson‘s Disease，PD)与肠道生物失调有关。然而，肠道菌群失调是否会导致运动并发症尚不清楚。对肠道微生物区系进行了16S核糖体RNA基因序列分析。统计分析肠道微生物区系的相对丰度及其与临床特征的关系。使用协方差分析(ANCOVA)来评估与疲劳或运动障碍相关的肠道微生物区系改变。47.5%的患者出现疲惫，21.9%的患者出现运动障碍。我们检测到98个属的细菌。帕金森病和运动并发症患者的肠道菌群有一定的变化。经过Bonferroni矫正后，疲劳症患者的肠道微生物区系不再有明显变化，而蓝乳杆菌的相对丰度降低(P<0.0001)，乳杆菌的相对丰度增加(P<0.0001)。两个混杂因素模型经方差分析调整后发现，年龄(p=0.0001)、病程(p=0.01)和衰弱(p=0.0004)是乳酸菌相对丰度降低的独立危险因素，而衰弱(p=0.009)是乳酸菌相对丰度增加的唯一独立危险因素。这表明肠道微生物区系的改变与晚期帕金森病患者运动并发症的发生有关。
IntroductionParkinson's disease (PD) is associated with gut dysbiosis. However, whether gut dysbiosis can cause motor complications is unclear.MethodsSubjects were enrolled from four independent movement disorder centers in Japan. We performed 16S ribosomal RNA gene sequence analysis of gut microbiota. Relative abundance of gut microbiota and relationships between them and clinical characteristics were statistically analyzed. Analysis of co-variance (ANCOVA) was used to assess altered gut microbiota associated with wearing-off or dyskinesia.ResultsWe enrolled 223 patients with PD. Wearing-off was noted in 47.5% of patients and dyskinesia in 21.9%. We detected 98 genera of bacteria. Some changes in the gut microbiota were observed in patients with PD and motor complications. After Bonferroni correction, patients with wearing-off showed decreased relative abundance of LachnospiraceaeBlautia (p < 0.0001) and increased relative abundance of LactobacillaceaeLactobacillus (p < 0.0001), but patients with dyskinesia no longer showed significant changes in the gut microbiota. Adjustment with two models of confounding factors followed by ANCOVA revealed that age (p < 0.0001), disease duration (p = 0.01), and wearing-off (p = 0.0004) were independent risks for the decreased relative abundance ofLachnospiraceae Blautia, and wearing-off (p = 0.009) was the only independent risk factor for the increased relative abundance ofLachnospiraceae Lactobacillus.ConclusionRelative abundance ofLachnospiraceae BlautiaandLactobacillaceae Lactobacilluswas significantly decreased and increased, respectively, in the gut microbiota of PD patients with motor complications. This indicates that an altered gut microbiota is associated with the development of motor complications in patients with advanced PD.