Pan-African polyspecific antivenom produced by caprylic acid purification of horse IgG:: an alternative to the antivenom crisis in Africa
Pan-African polyspecific antivenom produced by caprylic acid purification of horse IgG:: an alternative to the antivenom crisis in Africa
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DOI：
10.1016/j.trstmh.2004.09.014
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发表时间：
2005-06-01
影响因子：
2.2
通讯作者：
Rojas, G
中科院分区：
文献类型：
--
作者：
Gutiérrez, JM;Rojas, E;Rojas, G
一种多特异性泛非洲抗蛇毒血清是用非洲撒哈拉以南地区三种医学上最重要的蛇--Echis ocellatus、Bitis arietans和Naja nigricollis的毒液混合免疫的马的血浆制成的。该抗蛇毒血清是一种完整的免疫球蛋白制剂，通过辛酸沉淀非免疫球蛋白血浆蛋白而获得。该抗蛇毒血清有效地中和了免疫中使用的三种蛇毒的最重要的毒性活性，包括在检测前预先孵育蛇毒和抗蛇毒血清。这种抗蛇毒血清在中和棘球绦虫毒液中的毒素方面优于非洲设计的其他抗蛇毒血清。马高免血浆辛酸分级法是一种简单、方便、廉价的制备高质量全抗血清的方法。它构成了一项潜在的有价值的技术，可缓解非洲严重缺乏抗蛇毒血清的问题。(C)2005年皇家热带医学和卫生学会。爱思唯尔有限公司出版。保留所有权利。
A polyspecific Pan-African antivenom has been produced from the plasma of horses immunized with a mixture of the venoms of Echis ocellatus, Bitis arietans and Naja nigricollis, the three most medically important snakes in sub-Saharan Africa. The antivenom is a whole IgG preparation, obtained by caprylic acid precipitation of non-IgG plasma proteins. The antivenom effectively neutralizes the most important toxic activities of the three venoms used in the immunization in standard assays involving preincubation of venom and antivenom before testing. This antivenom compares favourably with other antivenoms designed for use in Africa with respect to neutralization of the toxins present in the venom of E. ocellatus. Caprylic acid fractionation of horse hyperimmune plasma is a simple, convenient and cheap protocol for the manufacture of high quality whole IgG antivenoms. It constitutes a potentially valuable technology for the alleviation of the critical shortage of antivenom in Africa. (c) 2005 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.