Interactions between 2-Cys peroxiredoxins and ascorbate in autophagosome formation during the heat stress response in Solanum lycopersicum.
Interactions between 2-Cys peroxiredoxins and ascorbate in autophagosome formation during the heat stress response in Solanum lycopersicum.
复制标题
番茄热应激反应期间自噬体形成中 2-Cys 过氧化还原蛋白和抗坏血酸之间的相互作用
DOI：
10.1093/jxb/erw013
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发表时间：
2016-03
影响因子：
6.9
通讯作者：
Foyer CH
中科院分区：
文献类型：
--
作者：
Cheng F;Yin LL;Zhou J;Xia XJ;Shi K;Yu JQ;Zhou YH;Foyer CH
2-半胱氨酸过氧化物酶通过与抗坏血酸依赖性途径和自噬的相互作用在番茄的热胁迫耐受性中发挥关键作用。2-半胱氨酸过氧化物酶（2-CP）的功能，在清除过氧化氢和脂质过氧化物，但其确切的作用，诱导自噬尚未得到表征。在这里，我们表明，热应激，这是已知的诱导氧化应激，导致同时积累的转录编码2-CP和自噬蛋白，以及自噬体，在番茄（番茄）植物。病毒诱导的番茄过氧化物酶基因2-CP 1、2-CP 2和2-CP 1/2的基因沉默导致番茄植物对热胁迫的敏感性增加。沉默2-CP 2或2-CP 1/2增加了与抗坏血酸生物合成相关的转录水平，但在没有应激的情况下对谷胱甘肽库没有影响。然而，与水-水循环相关的转录本的热诱导积累受到2-CP 1/2功能丧失的损害。自噬相关基因ATG 5和ATG 7的转录水平在2-CP 1/2功能受损的植物中更高，自噬体的形成增加，同时氧化和不溶性蛋白质的积累。ATG 5或ATG 7的沉默增加了2-CP转录本和蛋白质的水平，但降低了热胁迫耐受性。这些结果表明，2-CP履行在番茄热胁迫耐受性的关键作用，通过相互作用与抗坏血酸依赖的途径和自噬。
2-Cys peroxiredoxins fulfil a pivotal role in heat stress tolerance in Solanum lycopersicum via interactions with ascorbate-dependent pathways and autophagy. 2-Cys peroxiredoxins (2-CPs) function in the removal of hydrogen peroxide and lipid peroxides but their precise roles in the induction of autophagy have not been characterized. Here we show that heat stress, which is known to induce oxidative stress, leads to the simultaneous accumulation of transcripts encoding 2-CPs and autophagy proteins, as well as autophagosomes, in tomato (Solanum lycopersicum) plants. Virus-induced gene silencing of the tomato peroxiredoxin genes 2-CP1, 2-CP2, and 2-CP1/2 resulted in an increased sensitivity of tomato plants to heat stress. Silencing 2-CP2 or 2-CP1/2 increased the levels of transcripts associated with ascorbate biosynthesis but had no effect on the glutathione pool in the absence of stress. However, the heat-induced accumulation of transcripts associated with the water-water cycle was compromised by the loss of 2-CP1/2 functions. The transcript levels of autophagy-related genes ATG5 and ATG7 were higher in plants with impaired 2-CP1/2 functions, and the formation of autophagosomes increased, together with an accumulation of oxidized and insoluble proteins. Silencing of ATG5 or ATG7 increased the levels of 2-CP transcripts and protein but decreased heat stress tolerance. These results demonstrate that 2-CPs fulfil a pivotal role in heat stress tolerance in tomato, via interactions with ascorbate-dependent pathways and autophagy.