Genome-wide RNAi screen for host factors required for intracellular bacterial infection
Genome-wide RNAi screen for host factors required for intracellular bacterial infection
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DOI：
10.1126/science.1116008
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发表时间：
2005-08-19
期刊：
影响因子：
56.9
通讯作者：
Higgins, DE
中科院分区：
文献类型：
--
作者：
Agaisse, H;Burrack, LS;Higgins, DE
大多数宿主-病原体相互作用的研究都集中在病原体特异性毒力决定因子上。在这里，我们报告了一个全基因组RNA干扰筛选，以确定细胞内细菌致病所需的宿主因子。利用果蝇细胞和胞质病原体单核细胞增生李斯特菌，我们鉴定了305个双链RNA，这些RNA靶向广泛的细胞功能，改变了单核细胞增生李斯特菌。单核细胞增多症与偶然分枝杆菌（一种空泡病原体）进行的类似筛选比较，确定了可能在细胞内发病机制中发挥一般作用的宿主因子和特异性影响L.单核细胞增多症。
Most studies of host-pathogen interactions have focused on pathogen-specific virulence determinants. Here, we report a genome-wide RNA interference screen to identify host factors required for intracellular bacterial pathogenesis. Using Drosophila cells and the cytosolic pathogen Listeria monocytogenes, we identified 305 double-stranded RNAs targeting a wide range of cellular functions that altered L. monocytogenes infection. Comparison to a similar screen with Mycobacterium fortuitum, a vacuolar pathogen, identified host factors that may play a general role in intracellular pathogenesis and factors that specifically affect access to the cytosol by L. monocytogenes.