Non-invasive measurement of organ density in a rat simulated microgravity model.
Non-invasive measurement of organ density in a rat simulated microgravity model.
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发表时间：
2000-09
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通讯作者：
J. Nathan;B. Bradshaw;N. Bartoletti;M. Witten
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作者：
J. Nathan;B. Bradshaw;N. Bartoletti;M. Witten
航天飞行已经引起了对宇航员在太空中和在地球上执行任务后的健康和福祉的严重关注。微重力对健康的有害影响尚未得到很好的理解，尽管这些影响可能是由多种因素引起的，但体内的液体转移可能起着重要作用。假设我们认为，45 ℃的HDT将导致显着的变化，在1小时的实验时间内通过螺旋计算机断层扫描测量器官密度。方法雄性Fischer 344大鼠20只，随机分为实验组和对照组。实验组（n = 12）进行45 °头低位微重力暴露（45 ° HDT）1 h，对照组（n = 8）保持俯卧位1 h。在1小时结束时，使用螺旋计算机断层扫描（SCT）测量脑、肺、心脏、肝脏和左右肾的密度，同时大鼠保持在其实验位置。结果除右肾外，45 ° HDT组所有器官的密度与对照组相比均有显著性变化（p < 0.05）。大脑显示出最大的百分比增加，为45.6%，而肺部显示出最小的变化，为8.7%。结论：我们得出结论，与俯卧对照组相比，雄性45度HDT大鼠的SCT测量器官密度显著增加。
BACKGROUND Spaceflight has caused serious concerns to the health and well-being of astronauts, both while in space and post-mission on Earth. The deleterious health effects due to microgravity are not well understood and though these effects may be caused by multiple factors, fluid shifts within the body may play a major role. HYPOTHESIS We believe that 45 degrees C HDT will cause significant changes in organ density as measured by spiral computed tomography in a 1-h experimental time period. METHODS There were 20 male Fischer 344 rats that were randomly selected to be in an experimental or control group. The experimental group (n = 12) was subjected to a 45 degrees head down tilt microgravity exposure (45 degrees HDT) for 1 h, and the control group (n = 8) remained in the prone position for the same amount of time. At the end of 1 h, the density of the brain, lungs, heart, liver, and left and right kidneys were measured using spiral computed tomography (SCT) while the rats remained in their experimental positions. RESULTS With the exception of the right kidney, we demonstrated that there was a significant change (p < 0.05) in the densities of all tested organs in the 45 degrees HDT group when compared with the control group. The brain showed the largest percent increase at 45.6% while the lungs showed the least amount of change at 8.7%. CONCLUSIONS We conclude there are significant increases in organ density, as measured by SCT, in male 45 degrees HDT rats compared with prone controls.