Lactobacillus fermentum BR11, a potential new probiotic, alleviates symptoms of colitis induced by dextran sulfate sodium (DSS) in rats
Lactobacillus fermentum BR11, a potential new probiotic, alleviates symptoms of colitis induced by dextran sulfate sodium (DSS) in rats
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DOI：
10.1016/j.ijfoodmicro.2006.09.018
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发表时间：
2007-03-20
影响因子：
5.4
通讯作者：
Howarth, Gordon S.
中科院分区：
文献类型：
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作者：
Geier, Mark S.;Butler, Ross N.;Howarth, Gordon S.
目前炎症性肠病（IBD）的治疗相对无效。最近，益生菌已成为许多胃肠道疾病（包括IBD）的潜在治疗方式。然而，很少有益生菌在体内进行了适当的临床前筛选。本研究比较了四种候选益生菌对葡聚糖硫酸钠（DSS）诱导的大鼠结肠炎的影响。将Sprague道利大鼠灌胃1 mL潜在益生菌（1 × 10（10）CFU/mL）或溶媒，每天两次，持续14天。测试的菌株是鼠李糖乳杆菌GG（LGG）、嗜热链球菌TH-4（TH-4）、乳双歧杆菌Bb 12（Bb 12）和发酵乳杆菌BR 11（BR 11）。从第7天到第14天，通过饮用水中的2%DSS诱导结肠炎。每天监测疾病活动指数（DAI），直到在第14天处死大鼠。与DSS+媒介物相比，DSS+ Bb 12和DSS+ BR 11中的DAI降低。与DSS+媒介物相比，DSS+ BR 11（10%）和DSS+LGG（10%）中结肠长度增加。DSS+ Bb 12和DSS+ BR 11防止了在DSS +媒介物、DSS + LGG和DSS + TH-4中明显的远端结肠隐窝增生。BR 11在减轻结肠炎症状方面最有效。Bb 12的作用最小，而TH-4不能预防DSS-结肠炎，LGG实际上加重了结肠炎的一些指标。进一步研究L.发酵菌BR 11。(C)2006 Elsevier B. V.保留所有权利。
Current treatments for inflammatory bowel disease (IBD) are relatively ineffective. Recently, probiotics have emerged as a potential treatment modality for numerous gastrointestinal disorders, including IBD. Few probiotics, however, have undergone appropriate preclinical screening in vivo. The current study compared the effects of four candidate probiotics on development of dextran sulfate sodium (DSS)-induced colitis in rats. Sprague Dawley rats were gavaged 1 mL of the potential probiotic (1 x 10(10) CFU/mL), or vehicle, twice daily for 14 days. Strains tested were Lactobacillus rhamnosus GG (LGG), Streptococcus thermophilus TH-4 (TH-4), Bifidobacterium lactis Bb12 (Bb12) and Lactobacillus fermentum BR11 (BR11). Colitis was induced from day 7 to 14 via administration of 2% DSS in drinking water. Disease activity index (DAI) was monitored daily until rats were killed at day 14. DAI decreased in DSS+Bb12 and DSS+BR11 compared to DSS+Vehicle. Colon length increased in DSS+BR11 (10%) and DSS+LGG (10%) compared to DSS+Vehicle. DSS+Bb12 and DSS+BR11 prevented the distal colon crypt hyperplasia evident in DSS + Vehicle, DSS + LGG and DSS + TH-4. BR11 was most effective at reducing colitic symptoms. Bb 12 had minimal effects, whilst TH-4 did not prevent DSS-colitis and LGG actually exacerbated some indicators of colitis. Further studies into the potential benefits of L. fermentum BR11 are indicated. (C) 2006 Elsevier B.V. All rights reserved.