Differential localization of GABAA receptor α and β subunits in the hamster retina and relationship with glutamic acid decarboxylase immunoreactivity
Differential localization of GABAA receptor α and β subunits in the hamster retina and relationship with glutamic acid decarboxylase immunoreactivity
复制标题
仓鼠视网膜中GABAA受体α和β亚基的差异定位及其与谷氨酸脱羧酶免疫反应性的关系
DOI：
10.1016/s0304-3940(98)00326-7
复制
发表时间：
1998
影响因子：
2.5
通讯作者：
K. Yung
中科院分区：
文献类型：
--
作者：
Y. L. Lee;E. Cho;K. Yung
为了确定 GABAAα 和 β 亚基在仓鼠视网膜中的细胞定位，使用针对两种受体亚基和谷氨酸脱羧酶的市售抗体在灌注固定的仓鼠视网膜中进行单免疫细胞化学和双免疫细胞化学。在内丛状层的两层以及内核层的无长突细胞和双极细胞的核周中发现了强烈的GABAAβ免疫反应性。相反，没有检测到 GABAAα 免疫反应性。除少数 GABAAβ 免疫反应性无长突细胞外，所有细胞均未发现谷氨酸脱羧酶免疫反应性。目前的结果表明，仓鼠视网膜不同神经元亚群中 GABAAα 和 β 亚基的定位存在差异。
In order to determine the cellular localization of GABAAα and β subunits in the hamster retina, single and double immunocytochemistry was performed in perfuse-fixed hamster retina using commercially-available antibodies against the two receptor subunits and glutamic acid decarboxylase. Strong GABAAβ immunoreactivity was found in two strata of the inner plexiform layer and in perikarya of amacrine cells and bipolar cells in the inner nuclear layer. In contrast, no GABAAα immunoreactivity was detected. All but a few of the GABAAβ-immunoreactive amacrine cells were found not to display glutamic acid decarboxylase immunoreactivity. The present results indicate that there is a differential localization of GABAAα and β subunits in different neuronal subpopulations in the hamster retina.